Literature DB >> 9219230

The use of RNA probes for the analysis of gene expression.

D Belin1.   

Abstract

The monomeric bacteriophage RNA polymerases allow the synthesis of virtually any RNA molecule in unlimited quantity. In this protocol, we describe the preparation of plasmid and PCR-derived templates. A basic transcription protocol is provided with several optional modifications. The use of RNA probes in Northern blot hybridization and in RNase protection assays is described. The relative advantages and pitfalls of these two methods to quantitatively detect mRNA targets are discussed.

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Year:  1997        PMID： 9219230     DOI： 10.1007/BF02761751

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  28 in total

1.  Improved synthesis of full-length RNA probe at reduced incubation temperatures.

Authors:  P A Krieg
Journal:  Nucleic Acids Res       Date:  1990-11-11       Impact factor: 16.971

2.  Synthesis of long, capped transcripts in vitro by SP6 and T7 RNA polymerases.

Authors:  J K Yisraeli; D A Melton
Journal:  Methods Enzymol       Date:  1989       Impact factor: 1.600

3.  Synthesis of small RNAs using T7 RNA polymerase.

Authors:  J F Milligan; O C Uhlenbeck
Journal:  Methods Enzymol       Date:  1989       Impact factor: 1.600

4.  Transcription initiation site selection and abortive initiation cycling of phage SP6 RNA polymerase.

Authors:  S C Nam; C W Kang
Journal:  J Biol Chem       Date:  1988-12-05       Impact factor: 5.157

5.  A critical assessment of the RNAse protection assay as a means of determining exon sizes.

Authors:  E T Lau; R Y Kong; K S Cheah
Journal:  Anal Biochem       Date:  1993-03       Impact factor: 3.365

6.  Transcription by SP6 RNA polymerase exhibits an ATP dependence that is influenced by promoter topology.

Authors:  D R Taylor; M B Mathews
Journal:  Nucleic Acids Res       Date:  1993-04-25       Impact factor: 16.971

7.  Cloning and expression of the gene for bacteriophage T7 RNA polymerase.

Authors:  P Davanloo; A H Rosenberg; J J Dunn; F W Studier
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

8.  Light and electron microscopic in situ hybridization: non-radioactive labeling and detection, double hybridization, and combined hybridization-immunocytochemistry.

Authors:  D Egger; M Troxler; K Bienz
Journal:  J Histochem Cytochem       Date:  1994-06       Impact factor: 2.479

9.  A new double labeling technique for combined in situ hybridization and immunohistochemical analysis.

Authors:  J Kriegsmann; G Keyszer; T Geiler; R E Gay; S Gay
Journal:  Lab Invest       Date:  1994-12       Impact factor: 5.662

10.  Single-stranded DNA 'blue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering.

Authors:  D A Mead; E Szczesna-Skorupa; B Kemper
Journal:  Protein Eng       Date:  1986 Oct-Nov
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  3 in total

1.  Transcriptional activation of ydeA, which encodes a member of the major facilitator superfamily, interferes with arabinose accumulation and induction of the Escherichia coli arabinose PBAD promoter.

Authors:  S Bost; F Silva; D Belin
Journal:  J Bacteriol       Date:  1999-04       Impact factor: 3.490

2.  Transcriptional response to DNA damage in the archaeon Sulfolobus solfataricus.

Authors:  Vincenzo Salerno; Alessandra Napoli; Malcolm F White; Mosè Rossi; Maria Ciaramella
Journal:  Nucleic Acids Res       Date:  2003-11-01       Impact factor: 16.971

3.  Human myoblast fusion requires expression of functional inward rectifier Kir2.1 channels.

Authors:  J Fischer-Lougheed; J H Liu; E Espinos; D Mordasini; C R Bader; D Belin; L Bernheim
Journal:  J Cell Biol       Date:  2001-05-14       Impact factor: 10.539
  3 in total

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