Primary cultured chondrocytes of different origins respond differently to bFGF and TGF-beta.

Responses of rib and ear chondrocytes to basic fibroblast growth factor (bFGF) and transforming growth factor-beta (TGF-beta) were investigated using high density primary culture isolated from the same rabbit. Degrees of tritiated thymidine, leucine, and proline incorporation were used as indicators of DNA, protein and collagen syntheses, respectively. 10 ng/ml bFGF increased thymidine, proline, and leucine incorporation into rib, but not ear, chondrocytes. 1 ng/ml TGF-beta enhanced thymidine incorporation into both chondrocytes but did not affect proline or leucine incorporation into the ear cells. When both growth factors were added simultaneously, both cells showed rises in syntheses of DNA, protein and collagen. Incorporation of [35S]sulfate, used as indicator of proteoglycan synthesis, was elevated by TGF-beta but was reduced by bFGF especially in the rib cells. This inhibitory effect of bFGF was not reversed by cotreatment with TGF-beta in both cell types. Thus, the origin and cellular differentiation states of chondrocytes seem to cause different responses to these growth factors.