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Literature DB >> 9212440

Expression and functional analysis of a hyperglycosylated glucoamylase in a parental host, Aspergillus awamori var. kawachi.

Abstract

A modified glucoamylase gene (glaA) with an extra Thr- and Ser-rich Gp-I domain (T. Semimaru, M. Goto, K. Furukawa, and S. Hayashida, Appl. Environ. Microbiol. 61:2885-2890, 1995) was introduced into a mutant parental host, Aspergillus awamori var. kawachi, in which the original glaA gene had been completely deleted and replaced with the hygromycin phosphotransferase gene. The modified glaA was successfully expressed and secreted. The modified glucoamylase possessed higher digestibility of raw corn starch and higher stabilities in response to heat and extreme pH.

Entities: Chemical Species

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Year: 1997 PMID： 9212440 PMCID： PMC168589 DOI： 10.1128/aem.63.7.2940-2943.1997

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

15 in total

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Authors: A Aleshin; A Golubev; L M Firsov; R B Honzatko
Journal: J Biol Chem Date: 1992-09-25 Impact factor: 5.157

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Journal: Gene Date: 1987 Impact factor: 3.688

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Journal: Mol Gen Genet Date: 1993-12

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Journal: Mol Cell Biol Date: 1983-08 Impact factor: 4.272

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Journal: Eur J Biochem Date: 1984-12-17

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Authors: W Tanner; L Lehle
Journal: Biochim Biophys Acta Date: 1987-04-27

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Authors: M Goto; T Semimaru; K Furukawa; S Hayashida
Journal: Appl Environ Microbiol Date: 1994-11 Impact factor: 4.792

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Authors: J Tilburn; C Scazzocchio; G G Taylor; J H Zabicky-Zissman; R A Lockington; R W Davies
Journal: Gene Date: 1983-12 Impact factor: 3.688

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Authors: H Lis; N Sharon
Journal: Eur J Biochem Date: 1993-11-15

10. Glucoamylase overexpression in Aspergillus niger: molecular genetic analysis of strains containing multiple copies of the glaA gene.

Authors: J C Verdoes; P J Punt; J M Schrickx; H W van Verseveld; A H Stouthamer; C A van den Hondel
Journal: Transgenic Res Date: 1993-03 Impact factor: 2.788

3 in total