Literature DB >> 9210585

Structures of genes encoding phospholipase A2 inhibitors from the serum of Trimeresurus flavoviridis snake.

I Nobuhisa1, M Deshimaru, T Chijiwa, K Nakashima, T Ogawa, Y Shimohigashi, Y Fukumaki, S Hattori, H Kihara, M Ohno.   

Abstract

Inhibitors (PLIs) against snake venom gland phospholipases A2 (PLA2s) have been found in their sera. A cDNA encoding a PLI from Trimeresurus flavoviridis (Tf, habu snake, Crotalinae) serum, cPLI-A, was isolated from the Tf liver cDNA library and sequenced. Northern blot analysis with cPLI-A showed that PLIs are expressed only in liver. Genes for PLIs, gPLI-A and gPLI-B, were isolated from the Tf genomic DNA library and their nucleotide (nt) sequences were determined. The genes consisted of four exons and three introns, and exon 4 encoded the carbohydrate recognition domain (CRD)-like motif. Comparison of the nt sequences between gPLI-A and gPLI-B showed that these genes are highly homologous, including introns, except that exon 3 is rich in nonsynonymous nt substitutions which are almost four times as frequent as synonymous nt substitutions. This evolutionary feature of PLI genes is different from that of venom gland PLA2 isozyme genes in which nonsynonymous nt substitutions are spread over the entire mature protein-coding region.

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Year:  1997        PMID： 9210585     DOI： 10.1016/s0378-1119(97)00024-3

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  8 in total

1.  Two phospholipase A2 inhibitors from the plasma of Cerrophidion (Bothrops) godmani which selectively inhibit two different group-II phospholipase A2 myotoxins from its own venom: isolation, molecular cloning and biological properties.

Authors:  S Lizano; Y Angulo; B Lomonte; J W Fox; G Lambeau; M Lazdunski; J M Gutiérrez
Journal:  Biochem J       Date:  2000-03-15       Impact factor: 3.857

2.  Interisland mutation of a novel phospholipase A2 from Trimeresurus flavoviridis venom and evolution of Crotalinae group II phospholipases A2.

Authors:  Takahito Chijiwa; Sachiko Hamai; Shoji Tsubouchi; Tomohisa Ogawa; Masanobu Deshimaru; Naoko Oda-Ueda; Shosaku Hattori; Hiroshi Kihara; Susumu Tsunasawa; Motonori Ohno
Journal:  J Mol Evol       Date:  2003-11       Impact factor: 2.395

3.  Identification of the B subtype of gamma-phospholipase A2 inhibitor from Protobothrops flavoviridis serum and molecular evolution of snake serum phospholipase A2 inhibitors.

Authors:  Shuhei So; Takahito Chijiwa; Naoki Ikeda; Ikuo Nobuhisa; Naoko Oda-Ueda; Shosaku Hattori; Motonori Ohno
Journal:  J Mol Evol       Date:  2008-03-04       Impact factor: 2.395

Review 4.  Alpha-type phospholipase A2 inhibitors from snake blood.

Authors:  Norival A Santos-Filho; Claudia T Santos
Journal:  J Venom Anim Toxins Incl Trop Dis       Date:  2017-03-23

Review 5.  Structurally Robust and Functionally Highly Versatile-C-Type Lectin (-Related) Proteins in Snake Venoms.

Authors:  Johannes A Eble
Journal:  Toxins (Basel)       Date:  2019-03-01       Impact factor: 4.546

6.  Unique structure (construction and configuration) and evolution of the array of small serum protein genes of Protobothrops flavoviridis snake.

Authors:  Takahito Chijiwa; Kento Inamaru; Ami Takeuchi; Marie Maeda; Kazuaki Yamaguchi; Hiroki Shibata; Shosaku Hattori; Naoko Oda-Ueda; Motonori Ohno
Journal:  Biosci Rep       Date:  2019-07-05       Impact factor: 3.840

7.  Molecular diversity of proteins in biological offense and defense systems.

Authors:  Tomohisa Ogawa
Journal:  Mol Divers       Date:  2006-11       Impact factor: 2.943

8.  Discovery of the Gene Encoding a Novel Small Serum Protein (SSP) of Protobothrops flavoviridis and the Evolution of SSPs.

Authors:  Kento Inamaru; Ami Takeuchi; Marie Maeda; Hiroki Shibata; Yasuyuki Fukumaki; Naoko Oda-Ueda; Shosaku Hattori; Motonori Ohno; Takahito Chijiwa
Journal:  Toxins (Basel)       Date:  2020-03-12       Impact factor: 4.546
  8 in total

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