Embryologic origin of various epithelial cell types in the thyroid gland of the rat.

Ventral pharyngeal outpocketings and ultimobranchial outpocketings from the 14-day-old fetus of the Fischer rat were isolated before they fused to form thyroid glands. The outpocketings were implanted into different kidney capsules of adult male Fischer rats, and were allowed to grow for several months. Transplants were then excised and examined by electron microscopy. The ultimobranchial outpocketing gave rise to two types of follicles. One contained ciliated cells, cells with an abundant agranular reticulum, U cells with basal hemidesmosomes, and two types of cells with secretory vesicles. The other contained C cells separated from the follicular lumen by a single flat fiber-containing cell. The ventral outpocketing formed typical thyroid epithelium making up the usual thyroid follicles differing from follicles in the thyroid in situ by the absence of C cells. These follicles were functional as determined by autoradiographic studies with 125I but differed from thyroid follicles in situ with respect to size distribution. The results suggest that (1) in the adult thyroid gland the C cell in the usual follicle is an ultimobranchial contribution and (2) the so-called ultimobranchial follicle is ultimobranchial in origin but that the typical thyroid epithelium in mixed follicles of U cells and typical thyroid epithelium is a ventral contribution. The reason for the absence in the thyroid gland in situ of the variety of other cell types observed in ultimobranchial transplants is unknown.