Literature DB >> 9207048

Avoidance of sulfur loss during ammonia treatment of oligonucleotide phosphorothioates.

C B Reese1, Q Song.   

Abstract

Sulfur loss during the unblocking of phosphorothioate analogues of oligonucleotides with concentrated aqueous ammonia can be completely suppressed by the addition of 2-mercaptoethanol.

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Year:  1997        PMID： 9207048      PMCID： PMC146813          DOI： 10.1093/nar/25.14.2943

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  4 in total

1.  Oligonucleoside phosphorothioates.

Authors:  G Zon
Journal:  Methods Mol Biol       Date:  1993

2.  Stability of oligodeoxynucleoside phosphorodithioates and phosphorothioates in aqueous ammonia.

Authors:  J T Kodra; J Kehler; O Dahl
Journal:  Nucleic Acids Res       Date:  1995-08-25       Impact factor: 16.971

3.  Use of 1,2,4-dithiazolidine-3,5-dione (DtsNH) and 3-ethoxy-1,2,4-dithiazoline-5-one (EDITH) for synthesis of phosphorothioate-containing oligodeoxyribonucleotides.

Authors:  Q Xu; K Musier-Forsyth; R P Hammer; G Barany
Journal:  Nucleic Acids Res       Date:  1996-05-01       Impact factor: 16.971

4.  Solution-phase synthesis of phosphorothioate oligodeoxynucleosides by the phosphotriester method.

Authors:  I Barber; J L Imbach; B Rayner
Journal:  Antisense Res Dev       Date:  1995
  4 in total
  1 in total

1.  Phosphorothioate oligonucleotide quantification by μ-liquid chromatography-mass spectrometry.

Authors:  Robert Erb; Katharina Leithner; Andreas Bernkop-Schnürch; Herbert Oberacher
Journal:  AAPS J       Date:  2012-07-18       Impact factor: 4.009
  1 in total

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