| Literature DB >> 9206336 |
D Deutsch1, E Chityat, M Hekmati, A Palmon, Y Farkash, L Dafni.
Abstract
A human cDNA, encoding for the 175-amino-acid human amelogenin, was prepared by RT PCR from tooth bud mRNA and sub-cloned into pGEX-KG expression plasmid for over-expression in E. coli. The expressed protein was characterized by SDS-PAGE Western blotting, and N-terminal amino acid sequencing.Entities:
Mesh：
Substances：
Year: 1996 PMID： 9206336 DOI： 10.1177/08959374960100021201
Source DB: PubMed Journal: Adv Dent Res ISSN： 0895-9374