Y Wang1, B Zhu. 1. Department of Histology and Embryology, Chongqing University of Medical Sciences.
Abstract
OBJECTIVE: To study the effect of angelica polysaccharide (AP) on proliferation and differentiation of hematopoietic progenitor cells for clarifying the hematonic mechanism of angelica sinensis. METHODS: The techniques of culture of hematopoietic progenitor cell and hematopoietic growth factor (HGF) assay were used. RESULTS: AP could obviously promote the proliferation and differentiation of BFU-E, CFU-E, CFU-GM and CFU-MK in healthy and aniemic mice. The culture media of splenocyte, macrophage, fibroblast and skeletal muscle treated with AP had much stronger stimulating effects on hematopoietic progenitor cells. CONCLUSIONS: AP may enhance hematopoiesis by stimulating directly and/or indirectly macrophages, fibroblasts, lymphocytes in hematopoietic inductive microenvironment and muscle tissue to secrete some HGF (Epo, GM-CSF, IL, and MK-CSF). This is one of the biological mechanisms for hematonic effect of angelica sinensis.
OBJECTIVE: To study the effect of angelica polysaccharide (AP) on proliferation and differentiation of hematopoietic progenitor cells for clarifying the hematonic mechanism of angelica sinensis. METHODS: The techniques of culture of hematopoietic progenitor cell and hematopoietic growth factor (HGF) assay were used. RESULTS: AP could obviously promote the proliferation and differentiation of BFU-E, CFU-E, CFU-GM and CFU-MK in healthy and aniemic mice. The culture media of splenocyte, macrophage, fibroblast and skeletal muscle treated with AP had much stronger stimulating effects on hematopoietic progenitor cells. CONCLUSIONS: AP may enhance hematopoiesis by stimulating directly and/or indirectly macrophages, fibroblasts, lymphocytes in hematopoietic inductive microenvironment and muscle tissue to secrete some HGF (Epo, GM-CSF, IL, and MK-CSF). This is one of the biological mechanisms for hematonic effect of angelica sinensis.
Authors: Chang Liu; Jianqin Li; Fan Yi Meng; Simon X Liang; Ruixia Deng; Chi Kong Li; N H Pong; Ching Po Lau; Sau Wan Cheng; Jie Yu Ye; Jian L Chen; S T Yang; Haixia Yan; Shilin Chen; Beng H Chong; Mo Yang Journal: BMC Complement Altern Med Date: 2010-12-21 Impact factor: 3.659