[Expression and significance of hepatitis B virus genes in human primary intrahepatic cholangiocarcinoma and its surrounding tissue].

In order to explore the significance of HBV in the pathogenesis of cholangiocarcinoma at molecular level, HBV DNA, X gene, pre-S gene, S gene and C gene in 40 cases of primary intrahepatic cholangiocarcinoma and its surrounding tissue were detected by in situ hybridization technique. The results showed that 33 cases (82.5%) were positive for HBV DNA, while 31 (77.5%), 26 (65.0%), 24 (60.0%) and 27 (67.5%) cases were positive for X gene, pre-S gene, S gene and C gene, respectively. The results of this study suggest that there exists a close relationship between human primary intrahepatic cholangiocarcinoma and HBV chronic persistent infection. The expression of X gene might play an important role in the pathogenesis of the primary intrahepatic cholangiocarcinoma.