| Literature DB >> 9203407 |
S Iqbal1, J Robinson, D Deere, J R Saunders, C Edwards, J Porter.
Abstract
Direct detection of Escherichia coli from polluted river water was achieved using polymerase chain reaction (PCR) amplification of the uid gene. Amplification using DNA from environmental samples resulted in non-specific DNA fragments. Specific amplification was achieved through use of the touch-down PCR procedure. Targeting the uidA structural region of the gene gave reproducibly better amplification than targeting the uidR regulatory region. The data demonstrate conditions for optimal specific detection.Entities:
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Year: 1997 PMID： 9203407 DOI： 10.1046/j.1472-765x.1997.00160.x
Source DB: PubMed Journal: Lett Appl Microbiol ISSN： 0266-8254 Impact factor: 2.858