| Literature DB >> 9202714 |
C Schütt1, B Fürll, F Stelter, R S Jack, S Witt.
Abstract
Chinese hamster ovary (CHO) cells transfected with various genes are widely used as adherent cell monolayers to produce recombinant proteins. In this report we present a new culture technique for CHO cells transfected with the vector pPOL-DHFR-CD14 using a minifermenter (miniPERM, Heraeus) for the production of recombinant human endotoxin receptor CD14 (rCD14). The transfectants were cultured for 12-17 days under serum-free conditions and formed spheroids. From this system we harvested supernatants containing up to 3.1 mg/ml recombinant CD14 (rCD14). This represents a 200-fold increase of rCD14 yield compared to conventional adherent CHO cell culture.Entities:
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Year: 1997 PMID： 9202714 DOI： 10.1016/s0022-1759(97)00027-6
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303