Literature DB >> 9201941

Cleavage of 16S rRNA within the ribosome by mRNA modified in the A-site codon with phenanthroline-Cu(II).

D J Bucklin1, M A van Waes, J M Bullard, W E Hill.   

Abstract

Cleavage of 16S rRNA was obtained through mRNA modified at position +5 with the chemical cleavage agent 1,10-o-phenanthroline. In the presence of Cu2+, and after addition of reducing agent to the modified mRNA-70S complex, cleavage of proximal nucleotides within the 16S rRNA occurred. Primer extension analysis of 16S rRNA fragments revealed that nucleotides 528-532, 1196, and 1396-1397 were cleaved. Nucleotides 1053-1055 were also cleaved but did not show the same level of specificity as the former. These results provide evidence that at some point in the translation process these regions are all within 15 A of position +5, the A-site codon, on the mRNA.

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Year:  1997        PMID： 9201941     DOI： 10.1021/bi9624954

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  3 in total

1.  Positions in the 30S ribosomal subunit proximal to the 790 loop as determined by phenanthroline cleavage.

Authors:  G W Muth; S P Hennelly; W E Hill
Journal:  RNA       Date:  1999-07       Impact factor: 4.942

2.  Analysis of the conformation of the 3' major domain of Escherichia coli16S ribosomal RNA using site-directed photoaffinity crosslinking.

Authors:  A Montpetit; C Payant; J M Nolan; L Brakier-Gingras
Journal:  RNA       Date:  1998-11       Impact factor: 4.942

3.  The role of the mitochondrial ribosome in human disease: searching for mutations in 12S mitochondrial rRNA with high disruptive potential.

Authors:  Paul M Smith; Joanna L Elson; Laura C Greaves; Saskia B Wortmann; Richard J T Rodenburg; Robert N Lightowlers; Zofia M A Chrzanowska-Lightowlers; Robert W Taylor; Antón Vila-Sanjurjo
Journal:  Hum Mol Genet       Date:  2013-10-02       Impact factor: 6.150
  3 in total

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