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Literature DB >> 9201694

Nuclease susceptibility of the rat liver satellite DNA-containing chromatin decreases with age.

Abstract

Nuclease susceptibility of the satellite DNA-containing chromatin of the liver of young (18 +/- 2 weeks) and old (100 +/- 5 weeks) rats was analysed using nick-translated rat 185 bp satellite I DNA fragment cloned in pBR322. With increasing concentration of DNaseI and micrococcal nuclease (MNase), multimeric forms of the satellite ladder gradually disappear in both the ages. The rate of disappearance is faster in young rats as compared to old ones. Such age-dependent decrease in the susceptibility of satellite DNA-containing chromatin reflects its condensation towards heterochromatization in old age.

Entities: Gene Species

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Year: 1997 PMID： 9201694 DOI： 10.1023/a:1006850701989

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

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References

14 in total

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Journal: Mol Cell Biol Date: 1986-03 Impact factor: 4.272

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Authors: Y L Sun; Y Z Xu; M Bellard; P Chambon
Journal: EMBO J Date: 1986-02 Impact factor: 11.598

Nuclease susceptibility of the rat liver satellite DNA-containing chromatin decreases with age.

Review 1. Chromatin structure and transcription.

2. Chromosomal subunits in active genes have an altered conformation.

Review 3. Nuclease hypersensitive sites in chromatin.

4. Base sequence and evolution of guinea-pig alpha-satellite DNA.

5. Subunit structure of chromatin.

6. Chromatin structure in neuronal and neuroglial cell nuclei as a function of age.

7. Structure of the nucleosome core particle at 7 A resolution.

8. Nucleosomal instability and induction of new upstream protein-DNA associations accompany activation of four small heat shock protein genes in Drosophila melanogaster.

9. DNase I hypersensitive sites of the 5' region of the fibronectin gene of the liver of the rat.

10. Digestion of the chicken beta-globin gene chromatin with micrococcal nuclease reveals the presence of an altered nucleosomal array characterized by an atypical ladder of DNA fragments.