| Literature DB >> 9201389 |
K Venugopal1, K Howes, G S Barron, L N Payne.
Abstract
We describe the construction of a recombinant baculovirus containing the cloned DNA encoding the gp85 envelope glycoprotein of HPRS-103 (subgroup J) avian leukosis virus fused to the carboxy-terminus of the affinity tag glutathione-S-transferase. The fusion protein was efficiently secreted into the supernatant medium of the infected insect cell culture and could be purified in a single step using immobilized glutathione. An enzyme-linked immunosorbent assay using the recombinant protein was found to be specific and sensitive for detection of HPRS-103 virus-specific antibodies in the sera of infected birds.Entities:
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Year: 1997 PMID: 9201389
Source DB: PubMed Journal: Avian Dis ISSN: 0005-2086 Impact factor: 1.577