Literature DB >> 9200122

Replication, expression and segregation of plasmid-borne DNA in genetically transformed malaria parasites.

M R van Dijk1, R Vinkenoog, J Ramesar, R A Vervenne, A P Waters, C J Janse.   

Abstract

To fully exploit the transfection technology developed for Plasmodium we investigated the features of replication, expression and segregation of an episomally maintained DNA construct during a sexual blood stage development in genetically transformed parasites of P. berghei. Using DNA in situ hybridisation techniques we were able to show that the introduced DNA construct is located in the nucleus of the parasite and is not segregating uniformly during schizogony. Replication of the construct mainly takes place between 16 and 24 h after invasion of the merozoites, coinciding with chromosomal replication. Furthermore the plasmid-borne DHFR/TS gene is constitutively transcribed throughout the asexual blood stage development. Hence the DHFR/TS promoter would appear to be a useful tool in the study of (over)expression of introduced genes and performing complementation studies in transfected parasites during the complete a sexual blood stage development of P. berghei.

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Year:  1997        PMID： 9200122     DOI： 10.1016/s0166-6851(97)02843-0

Source DB:  PubMed          Journal:  Mol Biochem Parasitol        ISSN： 0166-6851            Impact factor:   1.759


  15 in total

1.  An alteration in concatameric structure is associated with efficient segregation of plasmids in transfected Plasmodium falciparum parasites.

Authors:  R A O'Donnell; P R Preiser; D H Williamson; P W Moore; A F Cowman; B S Crabb
Journal:  Nucleic Acids Res       Date:  2001-02-01       Impact factor: 16.971

2.  Green fluorescent protein as a marker in Plasmodium berghei transformation.

Authors:  A A Sultan; V Thathy; V Nussenzweig; R Ménard
Journal:  Infect Immun       Date:  1999-05       Impact factor: 3.441

3.  Topology and replication of a nuclear episomal plasmid in the rodent malaria Plasmodium berghei.

Authors:  Donald H Williamson; Chris J Janse; Peter W Moore; Andrew P Waters; Peter R Preiser
Journal:  Nucleic Acids Res       Date:  2002-02-01       Impact factor: 16.971

Review 4.  Towards genome-wide experimental genetics in the in vivo malaria model parasite Plasmodium berghei.

Authors:  Joachim M Matz; Taco W A Kooij
Journal:  Pathog Glob Health       Date:  2015-03-19       Impact factor: 2.894

5.  A genetic screen for improved plasmid segregation reveals a role for Rep20 in the interaction of Plasmodium falciparum chromosomes.

Authors:  Rebecca A O'Donnell; Lúcio H Freitas-Junior; Peter R Preiser; Donald H Williamson; Manoj Duraisingh; Terry F McElwain; Artur Scherf; Alan F Cowman; Brendan S Crabb
Journal:  EMBO J       Date:  2002-03-01       Impact factor: 11.598

6.  CRISPR/Cas9 mediated sequential editing of genes critical for ookinete motility in Plasmodium yoelii.

Authors:  Cui Zhang; Han Gao; Zhenke Yang; Yuanyuan Jiang; Zhenkui Li; Xu Wang; Bo Xiao; Xin-Zhuan Su; Huiting Cui; Jing Yuan
Journal:  Mol Biochem Parasitol       Date:  2016-12-26       Impact factor: 1.759

7.  Functional identification of the Plasmodium centromere and generation of a Plasmodium artificial chromosome.

Authors:  Shiroh Iwanaga; Shahid M Khan; Izumi Kaneko; Zoe Christodoulou; Chris Newbold; Masao Yuda; Chris J Janse; Andrew P Waters
Journal:  Cell Host Microbe       Date:  2010-03-18       Impact factor: 21.023

8.  Transfected Plasmodium knowlesi produces bioactive host gamma interferon: a new perspective for modulating immune responses to malaria parasites.

Authors:  Hastings Ozwara; Jan A M Langermans; Clemens H M Kocken; Annemarie van der Wel; Peter H van der Meide; Richard A W Vervenne; Jason M Mwenda; Alan W Thomas
Journal:  Infect Immun       Date:  2003-08       Impact factor: 3.441

9.  Blood-stage Plasmodium infection induces CD8+ T lymphocytes to parasite-expressed antigens, largely regulated by CD8alpha+ dendritic cells.

Authors:  Rachel J Lundie; Tania F de Koning-Ward; Gayle M Davey; Catherine Q Nie; Diana S Hansen; Lei Shong Lau; Justine D Mintern; Gabrielle T Belz; Louis Schofield; Francis R Carbone; Jose A Villadangos; Brendan S Crabb; William R Heath
Journal:  Proc Natl Acad Sci U S A       Date:  2008-09-17       Impact factor: 11.205

10.  Transgenic expression of a mosquito-stage malarial protein, Pbs21, in blood stages of transformed Plasmodium berghei and induction of an immune response upon infection.

Authors:  G Margos; M R van Dijk; J Ramesar; C J Janse; A P Waters; R E Sinden
Journal:  Infect Immun       Date:  1998-08       Impact factor: 3.441
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