Literature DB >> 9199474

A monoclonal antibody directed against the 97-kilodalton gonococcal hemin-binding protein inhibits hemin utilization by Neisseria gonorrhoeae.

B C Lee1, S Levesque.   

Abstract

Neisseria gonorrhoeae expresses two hemin-binding proteins (HmBPs) of 97,000 and 44,000 in molecular weight. A murine monoclonal antibody (MAb) produced against the 97-kDa HmBP from N. gonorrhoeae PID543 specifically inhibited in a concentration-dependent manner the ability of hemin to promote growth. The anti-97-kDa HmBP MAb competitively inhibited binding of the 97-kDa HmBP to a hemin-agarose affinity column. In Western immunoblots, the MAb recognized the 97-kDa homologs from a limited survey of clinical gonococcal isolates. These results support the contention that the 97-kDa HmBP is involved in the gonococcal hemin acquisition pathway.

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Year:  1997        PMID： 9199474      PMCID： PMC175416          DOI： 10.1128/iai.65.7.2970-2974.1997

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  30 in total

1.  Isolation of haemin-binding proteins of Neisseria gonorrhoeae.

Authors:  B C Lee
Journal:  J Med Microbiol       Date:  1992-02       Impact factor: 2.472

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Authors:  H Towbin; T Staehelin; J Gordon
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Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

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Authors:  B R Oakley; D R Kirsch; N R Morris
Journal:  Anal Biochem       Date:  1980-07-01       Impact factor: 3.365

5.  SJ-GC, a modified complete medium for growth of Neisseria gonorrhoeae.

Authors:  R K Shockley; E E Coffee; K H Johnston
Journal:  J Clin Microbiol       Date:  1980-07       Impact factor: 5.948

6.  Affinity chromatography of heme-binding proteins: an improved method for the synthesis of hemin-agarose.

Authors:  K Tsutsui; G C Mueller
Journal:  Anal Biochem       Date:  1982-04       Impact factor: 3.365

7.  Gonococcal transferrin-binding protein 1 is required for transferrin utilization and is homologous to TonB-dependent outer membrane receptors.

Authors:  C N Cornelissen; G D Biswas; J Tsai; D K Paruchuri; S A Thompson; P F Sparling
Journal:  J Bacteriol       Date:  1992-09       Impact factor: 3.490

8.  Enzyme-linked immunosorbent assay compared with immunoprecipitation for serotyping Neisseria meningitidis and its use in demonstrating serotype polymorphism.

Authors:  E B Mackie; L A White; K N Brown; L E Bryan
Journal:  J Clin Microbiol       Date:  1981-03       Impact factor: 5.948

9.  The Neisseria meningitidis haemoglobin receptor: its role in iron utilization and virulence.

Authors:  I Stojiljkovic; V Hwa; L de Saint Martin; P O'Gaora; X Nassif; F Heffron; M So
Journal:  Mol Microbiol       Date:  1995-02       Impact factor: 3.501

10.  Hemin uptake system of Yersinia enterocolitica: similarities with other TonB-dependent systems in gram-negative bacteria.

Authors:  I Stojiljkovic; K Hantke
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  6 in total

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Authors:  Ching-Ju Chen; Dalton Mclean; Christopher E Thomas; James E Anderson; P Frederick Sparling
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Journal:  J Bacteriol       Date:  2004-02       Impact factor: 3.490

3.  Phase variation of hemoglobin utilization in Neisseria gonorrhoeae.

Authors:  C J Chen; C Elkins; P F Sparling
Journal:  Infect Immun       Date:  1998-03       Impact factor: 3.441

4.  The Neisseria meningitidis ZnuD zinc receptor contributes to interactions with epithelial cells and supports heme utilization when expressed in Escherichia coli.

Authors:  Pradeep Kumar; Soma Sannigrahi; Yih-Ling Tzeng
Journal:  Infect Immun       Date:  2011-11-14       Impact factor: 3.441

5.  Molecular characterization of the hemin uptake locus (hmu) from Yersinia pestis and analysis of hmu mutants for hemin and hemoprotein utilization.

Authors:  J M Thompson; H A Jones; R D Perry
Journal:  Infect Immun       Date:  1999-08       Impact factor: 3.441

6.  Neisseria gonorrhoeae heme biosynthetic mutants utilize heme and hemoglobin as a heme source but fail to grow within epithelial cells.

Authors:  P C Turner; C E Thomas; C Elkins; S Clary; P F Sparling
Journal:  Infect Immun       Date:  1998-11       Impact factor: 3.441
  6 in total

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