| Literature DB >> 9189830 |
R L Cleek1, A A Rege, L A Denner, S G Eskin, A G Mikos.
Abstract
We fabricated poly(DL-lactic-co-glycolic acid) (PLGA) 50:50 microparticles loaded with an antisense (AS) oligodeoxy-nucleotide (ODN) against the rat tenascin mRNA and determined the effect in vitro of the AS-ODN released on smooth muscle cell (SMC) proliferation and migration. AS-ODN was entrapped using a double-emulsion-solvent-extraction technique with high efficiency. Release of AS-ODN was characterized by a small initial-burst effect followed by a period of controlled AS-ODN release for up to 20 days. SMC proliferation studies exhibited dose-dependent growth inhibition with AS-ODN-loaded microparticles. Microparticles loaded with scrambled (SC) ODN showed less growth inhibition than AS-ODN. Moreover, only the AS-ODN-loaded microparticles inhibited migration. These results demonstrate the feasibility of entrapping an AS-ODN to rat tenascin in PLGA microparticles for controlled delivery to inhibit SMC proliferation and migration.Entities:
Keywords: Non-programmatic
Mesh：
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Year: 1997 PMID： 9189830 DOI： 10.1002/(sici)1097-4636(19970615)35:4<525::aid-jbm12>3.0.co;2-a
Source DB: PubMed Journal: J Biomed Mater Res ISSN： 0021-9304