Literature DB >> 9188526

Requirement of guanosine triphosphate-bound ran for signal-mediated nuclear protein export.

S A Richards1, K L Carey, I G Macara.   

Abstract

A leucine-rich nuclear export signal (NES) allows rapid export of proteins from cell nuclei. Microinjection studies revealed a role for the guanosine triphosphatase (GTPase) Ran in NES-mediated export. Nuclear injection of a Ran mutant (Thr24 --> Asn) blocked protein export but not import, whereas depletion of the Ran nucleotide exchange factor RCC1 blocked protein import but not export. However, injection of Ran GTPase-activating protein (RanGAP) into RCC1-depleted cell nuclei inhibited export. Coinjection with Ran mutants insensitive to RanGAP prevented this inhibition. Therefore, NES-mediated protein export appears to require a Ran-GTP complex but does not require Ran-dependent GTP hydrolysis.

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Year:  1997        PMID： 9188526     DOI： 10.1126/science.276.5320.1842

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  71 in total

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