W Wigger-Alberti1, P Elsner. 1. Department of Dermatology, University Hospital, Zurich, Switzerland.
Abstract
BACKGROUND:Protective creams (PCs) have been studied in different models indicating a protective effect of some products. In order to compare PCs in different studies, a generic reference standard should be available. OBJECTIVE: The purpose of this study was to investigate if petrolatum prevents epidermal barrier disruption induced by various irritants in a repetitive irritation test (RIT) and to assess its potential as a standard reference product. METHODS: White petrolatum was evaluated against a set of 4 irritants [10% sodium lauryl sulphate (SLS), 1% sodium hydroxide (NaOH), 30% lactic acid (LA) and undiluted toluene (TOL)] in the RIT. Twenty subjects were tested on the paravertebral skin of the midback. Irritation was assessed by visual scoring, transepidermal water loss and colorimetry. RESULTS: Petrolatum was very effective against SLS, NaOH and LA irritation, and it provided a moderate protection against TOL. CONCLUSION: Petrolatum can be recommended as a standard reference substance against which PCs may be compared as it is effective against water-soluble and water-insoluble irritants in a standardized test procedure.
RCT Entities:
BACKGROUND: Protective creams (PCs) have been studied in different models indicating a protective effect of some products. In order to compare PCs in different studies, a generic reference standard should be available. OBJECTIVE: The purpose of this study was to investigate if petrolatum prevents epidermal barrier disruption induced by various irritants in a repetitive irritation test (RIT) and to assess its potential as a standard reference product. METHODS:White petrolatum was evaluated against a set of 4 irritants [10% sodium lauryl sulphate (SLS), 1% sodium hydroxide (NaOH), 30% lactic acid (LA) and undiluted toluene (TOL)] in the RIT. Twenty subjects were tested on the paravertebral skin of the midback. Irritation was assessed by visual scoring, transepidermal water loss and colorimetry. RESULTS:Petrolatum was very effective against SLS, NaOH and LA irritation, and it provided a moderate protection against TOL. CONCLUSION:Petrolatum can be recommended as a standard reference substance against which PCs may be compared as it is effective against water-soluble and water-insoluble irritants in a standardized test procedure.
Authors: Eric L Simpson; Joanne R Chalmers; Jon M Hanifin; Kim S Thomas; Michael J Cork; W H Irwin McLean; Sara J Brown; Zunqiu Chen; Yiyi Chen; Hywel C Williams Journal: J Allergy Clin Immunol Date: 2014-10 Impact factor: 10.793