Literature DB >> 9185271

Comparison of adult somatic and excretory-secretory antigens in enzyme-linked immunosorbent assay for serodiagnosis of human infection with Fasciola gigantica.

W Maleewong1, P M Intapan, C Wongkham, K Tomanakan, W Daenseekaew, W Sukeepaisarnjaroen.   

Abstract

Adult somatic antigen extract of Fasciola gigantica was compared with excretory-secretory (ES) antigen in an enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of human fascioliasis gigantica. The absorbance values in ELISA using the adult somatic antigen were not significantly different from the values obtaining using ES antigen (p > 0.05). The diagnostic sensitivity, specificity and positive and negative predictive values of the test using adult somatic extract as antigen were 100%, 98%, 70% and 100%, respectively. On the other hand, these values of the test using adult ES antigen were 100%, 99.3%, 87.5% and 100%, respectively. It appears that both somatic and ES antigens are effective antigens for use in the serodiagnosis of human fascioliasis gigantica.

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Year:  1996        PMID： 9185271

Source DB:  PubMed          Journal:  Southeast Asian J Trop Med Public Health        ISSN： 0125-1562            Impact factor:   0.267


  6 in total

1.  Development and evaluation of a dipstick assay in diagnosis of human fasciolosis.

Authors:  Nehad Mahmoud Ali
Journal:  Parasitol Res       Date:  2011-10-21       Impact factor: 2.289

2.  Evaluation of immunoglobulin G subclass antibodies against recombinant Fasciola gigantica cathepsin L1 in an enzyme-linked immunosorbent assay for serodiagnosis of human fasciolosis.

Authors:  Chaisiri Wongkham; Chairat Tantrawatpan; Pewpan M Intapan; Wanchai Maleewong; Sopit Wongkham; Kunio Nakashima
Journal:  Clin Diagn Lab Immunol       Date:  2005-10

3.  Monoclonal antibody against recombinant Fasciola gigantica cathepsin L1H could detect juvenile and adult cathepsin Ls of Fasciola gigantica.

Authors:  Sirima Wongwairot; Pornanan Kueakhai; Narin Changklungmoa; Wipaphorn Jaikua; Veerawat Sansri; Krai Meemon; Sineenart Songkoomkrong; Suda Riengrojpitak; Prasert Sobhon
Journal:  Parasitol Res       Date:  2014-10-17       Impact factor: 2.289

4.  Effectiveness of Fasciola gigantica excretory-secretory and recombinant cathepsin L antigens for rapid diagnosis of human fascioliasis using immunochromatographic devices.

Authors:  Lakkhana Sadaow; Hiroshi Yamasaki; Yasuyuki Morishima; Oranuch Sanpool; Rutchanee Rodpai; Penchom Janwan; Patcharaporn Boonroumkaew; Wanchai Maleewong; Pewpan M Intapan
Journal:  Parasitol Res       Date:  2020-10-03       Impact factor: 2.289

Review 5.  Recent Developments in Recombinant Proteins for Diagnosis of Human Fascioliasis.

Authors:  Abolfazl Mirzadeh; Farid Jafarihaghighi; Elham Kazemirad; Shokouh Shahrokhi Sabzevar; Mohammad Hossein Tanipour; Mehdi Ardjmand
Journal:  Acta Parasitol       Date:  2020-09-24       Impact factor: 1.440

6.  Dot-blot immunoassay of Fasciola gigantica infection using 27 kDa and adult worm regurge antigens in Egyptian patients.

Authors:  Hanan H Kamel; Ghada A Saad; Rania M Sarhan
Journal:  Korean J Parasitol       Date:  2013-04-25       Impact factor: 1.341
  6 in total

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