Literature DB >> 917038

A quantitative assay of mutation induction at the hypoxanthine-guanine phosphoribosyl transferase locus in Chinese hamster ovary cells (CHO/HGPRT system): development and definition of the system.

J P O'Neill, P A Brimer, R Machanoff, G P Hirsch, A W Hsie.   

Abstract

An assay is described for the measurement of mutation induction at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in Chinese hamster ovary (CHO) cells utilizing resistance to 6-thioguanine (TG). Optimal selection conditions are defined for such parameters as phenotypic expression time prior to selection, and TG concentration and cell density which permits maximum mutant recovery. The nature of the TG-resistant mutants is characterized by several physiological and biochemical methods. The data demonstrate that more than 98% of the mutant clones isolated by this selection procedure contain altered HGPRTase activity. The CHO/HGPRT system thus shows the specificity necessary for a specific gene locus mutational assay.

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Year:  1977        PMID： 917038     DOI： 10.1016/0027-5107(77)90047-1

Source DB:  PubMed          Journal:  Mutat Res        ISSN： 0027-5107            Impact factor:   2.433


  16 in total

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Authors:  H Ikehata; S Kaneda; F Yamao; T Seno; T Ono; F Hanaoka
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2.  Medicinal plants in therapy.

Authors:  N R Farnsworth; O Akerele; A S Bingel; D D Soejarto; Z Guo
Journal:  Bull World Health Organ       Date:  1985       Impact factor: 9.408

3.  Detection of deletion mutations in pSV2gpt-transformed cells.

Authors:  K R Tindall; L F Stankowski; R Machanoff; A W Hsie
Journal:  Mol Cell Biol       Date:  1984-07       Impact factor: 4.272

4.  Cell cycle effects and cellular pharmacology of 5-aza-2'-deoxycytidine.

Authors:  R L Momparler; J Samson; L F Momparler; G E Rivard
Journal:  Cancer Chemother Pharmacol       Date:  1984       Impact factor: 3.333

5.  Postreplication repair and the susceptibility of Chinese hamster cells to cytotoxic and mutagenic effects of alkylating agents.

Authors:  J Friedman; E Huberman
Journal:  Proc Natl Acad Sci U S A       Date:  1980-10       Impact factor: 11.205

6.  Isolation and characterization of mouse FM3A cell mutants which are devoid of Newcastle disease virus receptors.

Authors:  T Hara; S Hattori; M Kawakita
Journal:  J Virol       Date:  1989-01       Impact factor: 5.103

7.  Identification of 7,12-dimethylbenz[a]anthracene metabolites that lead to mutagenesis in mammalian cells.

Authors:  E Huberman; M W Chou; S K Yang
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

8.  The influence of repair pathways on the cytotoxicity and mutagenicity induced by the pyridyloxobutylation pathway of tobacco-specific nitrosamines.

Authors:  Li Li; Joana Perdigao; Anthony E Pegg; Yanbin Lao; Stephen S Hecht; Bruce R Lindgren; Joyce T Reardon; Aziz Sancar; Elizabeth V Wattenberg; Lisa A Peterson
Journal:  Chem Res Toxicol       Date:  2009-08       Impact factor: 3.739

9.  Acute, multiple-dose dermal and genetic toxicity of Nu-3: a novel antimicrobial agent.

Authors:  Juan Sun; Yanxin Hu; Shanping Cao; Guozhong Zhang; Lun-Quan Sun; Ming Wang
Journal:  J Biomed Biotechnol       Date:  2010-07-20

10.  Role of aldehydes in the toxic and mutagenic effects of nitrosamines.

Authors:  Lisa A Peterson; Anna M Urban; Choua C Vu; Meredith E Cummings; Lee C Brown; Janel K Warmka; Li Li; Elizabeth V Wattenberg; Yesha Patel; Daniel O Stram; Anthony E Pegg
Journal:  Chem Res Toxicol       Date:  2013-09-25       Impact factor: 3.739
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