Changes in BRCA2 expression during progression of the cell cycle.

It has been shown that genetic alterations in BRCA1 and BRCA2 can predispose an individual to develop breast cancer. We investigated the expression of both BRCA2 and BRCA1 during the progression of the cell cycle by northern blot analysis. In MCF-10F (normal breast epithelial cell line) and MCF-7 (breast cancer cell line) cells the expression of BRCA2 RNA was low in G0 and early G1 phases then up-regulated at the G1/S phase junction. Expression of BRCA2 was maintained at relatively high levels when cells progressed through S and G2/M phases. For MCF-7 cells, the level of BRCA2 transcript decreased as cells were released from nocodazole-mediated metaphase arrest. This is consistent with the observation of low but detectable BRCA2 RNA level in G1 phase of the cell cycle. For both cell lines, the patterns of RNA expression of BRCA1 and BRCA2 were similar during the proliferation phase of cell cycle. However, the transcripts from both genes were undetectable in quiescent cells. These results suggest important functions for both BRCA2 and BRCA1 in regulation of cell growth.