Literature DB >> 9168121

A bacterial cloning vector using a mutated Aequorea green fluorescent protein as an indicator.

S Inouye1, H Ogawa, K Yasuda, K Umesono, F I Tsuji.   

Abstract

The bacterial cloning vector, pGreenscript A, derived from the mutated Aequorea green fluorescent protein (GFP-S65A) gene, when expressed in E. coli produced colonies that showed yellow color under daylight and strong green fluorescence under long-wave ultraviolet light. The vector was used to select for inserted foreign genes based on the loss of the yellow color/green fluorescence of E. coli cells caused by the insertional inactivation of GFP production.

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Year:  1997        PMID： 9168121     DOI： 10.1016/s0378-1119(96)00753-6

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  10 in total

1.  Neocortical origin and tangential migration of guidepost neurons in the lateral olfactory tract.

Authors:  N Tomioka; N Osumi; Y Sato; T Inoue; S Nakamura; H Fujisawa; T Hirata
Journal:  J Neurosci       Date:  2000-08-01       Impact factor: 6.167

2.  Development of a suicidal vector-cloning system based on butanal susceptibility due to an expression of YqhD aldehyde reductase.

Authors:  Changhan Lee; Chankyu Park
Journal:  J Microbiol       Date:  2012-04-27       Impact factor: 3.422

3.  White and green screening with circular polymerase extension cloning for easy and reliable cloning.

Authors:  Elizabeth B Speltz; Lynne Regan
Journal:  Protein Sci       Date:  2013-05-08       Impact factor: 6.725

4.  Mammalian ELAV-like neuronal RNA-binding proteins HuB and HuC promote neuronal development in both the central and the peripheral nervous systems.

Authors:  W Akamatsu; H J Okano; N Osumi; T Inoue; S Nakamura; S Sakakibara; M Miura; N Matsuo; R B Darnell; H Okano
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-17       Impact factor: 11.205

5.  Direct facile screening of recombinant DNA vector constructs.

Authors:  Paul T Winnard; Rushi Challa; Zaver M Bhujwalla; Venu Raman
Journal:  Anal Biochem       Date:  2014-01-03       Impact factor: 3.365

6.  A novel prokaryotic vector for identification and selection of recombinants: direct use of the vector for expression studies in E. coli.

Authors:  Sampali Banerjee; Jitendra Kumar; Anjali Apte-Deshpande; Sriram Padmanabhan
Journal:  Microb Cell Fact       Date:  2010-05-11       Impact factor: 5.328

7.  Aequorin variants with improved bioluminescence properties.

Authors:  E Dikici; X Qu; L Rowe; L Millner; C Logue; S K Deo; M Ensor; S Daunert
Journal:  Protein Eng Des Sel       Date:  2009-01-23       Impact factor: 1.650

8.  A dual-fluorescence reporter system for high-throughput clone characterization and selection by cell sorting.

Authors:  Juno Choe; Haiwei H Guo; Ger van den Engh
Journal:  Nucleic Acids Res       Date:  2005-03-14       Impact factor: 16.971

9.  Replacing Standard Reporters from Molecular Cloning Plasmids with Chromoproteins for Positive Clone Selection.

Authors:  Margarita Daniela Tafoya-Ramírez; Felipe Padilla-Vaca; Ana Patricia Ramírez-Saldaña; Josué Daniel Mora-Garduño; Ángeles Rangel-Serrano; Naurú Idalia Vargas-Maya; Luz Janeth Herrera-Gutiérrez; Bernardo Franco
Journal:  Molecules       Date:  2018-05-31       Impact factor: 4.411

10.  The evolution of basal progenitors in the developing non-mammalian brain.

Authors:  Tadashi Nomura; Chiaki Ohtaka-Maruyama; Wataru Yamashita; Yoshio Wakamatsu; Yasunori Murakami; Federico Calegari; Kunihiro Suzuki; Hitoshi Gotoh; Katsuhiko Ono
Journal:  Development       Date:  2016-01-01       Impact factor: 6.868
  10 in total

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