BACKGROUND: To study the metastatic behavior of human prostate cancer cell lines, orthotopic injection in nude mice was performed. METHODS: Local tumor growth, metastasis formation, prostate-specific antigen, and androgen receptor expression were examined. RESULTS: Hormone-independent cell lines (PC-3, PC-3-125-IL, and TSU-Pr1) were highly tumorigenic and had a higher rate of lymph node metastasis after orthotopic than after subcutaneous implantation. PC-3 cell lines also consistently metastasized to the lungs. The androgen-sensitive LNCaP cell line showed local growth in 7 of 10, and lymph node metastasis in 4 animals. Significant serum PSA levels and strong receptor expression in primary and metastatic tumor tissues were observed. CONCLUSIONS: These results demonstrates that orthotopic implantation of human prostate cancer cell lines, including LNCaP, reproducibly leads to metastasis formation in nude mice.
BACKGROUND: To study the metastatic behavior of humanprostate cancer cell lines, orthotopic injection in nude mice was performed. METHODS: Local tumor growth, metastasis formation, prostate-specific antigen, and androgen receptor expression were examined. RESULTS: Hormone-independent cell lines (PC-3, PC-3-125-IL, and TSU-Pr1) were highly tumorigenic and had a higher rate of lymph node metastasis after orthotopic than after subcutaneous implantation. PC-3 cell lines also consistently metastasized to the lungs. The androgen-sensitive LNCaP cell line showed local growth in 7 of 10, and lymph node metastasis in 4 animals. Significant serum PSA levels and strong receptor expression in primary and metastatic tumor tissues were observed. CONCLUSIONS: These results demonstrates that orthotopic implantation of humanprostate cancer cell lines, including LNCaP, reproducibly leads to metastasis formation in nude mice.
Authors: Mahesh Alur; Minh M Nguyen; Scott E Eggener; Feng Jiang; Soheil S Dadras; Jeffrey Stern; Simon Kimm; Kim Roehl; James Kozlowski; Michael Pins; Marek Michalak; Rajiv Dhir; Zhou Wang Journal: Am J Pathol Date: 2009-07-16 Impact factor: 4.307
Authors: Flávia R O Silva; Camila T Nabeshima; Maria H Bellini; Nestor Schor; Nilson D Vieira; Lilia C Courrol Journal: J Fluoresc Date: 2012-09-09 Impact factor: 2.217
Authors: Elizabeth C Wayne; Siddarth Chandrasekaran; Michael J Mitchell; Maxine F Chan; Rachel E Lee; Chris B Schaffer; Michael R King Journal: J Control Release Date: 2015-12-28 Impact factor: 9.776
Authors: Hannes Steiner; Sonia Godoy-Tundidor; Hermann Rogatsch; Andreas P Berger; Dietmar Fuchs; Barbara Comuzzi; Georg Bartsch; Alfred Hobisch; Zoran Culig Journal: Am J Pathol Date: 2003-02 Impact factor: 4.307