Literature DB >> 9163501

A novel method for isolation of Chlamydia pneumoniae by treatment with trypsin or EDTA.

Y Kazuyama1, S M Lee, K Amamiya, F Taguchi.   

Abstract

To establish a novel method for the efficient isolation of Chlamydia pneumoniae, experiments were performed to determine the effects of EDTA or trypsin treatment of C. pneumoniae on its adsorption and inclusion body formation. Treatment of C. pneumoniae with 0.1% trypsin or 1 mM EDTA significantly increased inclusion body-forming activity from 8,000- to 10,000-fold higher than that of the control. C. pneumoniae was successfully isolated in cultured cells which were inoculated with clinical specimens after treatment with 0.1% trypsin.

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Year:  1997        PMID： 9163501      PMCID： PMC229806          DOI： 10.1128/jcm.35.6.1624-1626.1997

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Isolation of Chlamydia pneumoniae from middle ear aspirates of otitis media with effusion: a case report.

Authors:  H Ogawa; T Fujisawa; Y Kazuyama
Journal:  J Infect Dis       Date:  1990-10       Impact factor: 5.226

2.  Partial amino acid sequence and molecular cloning of the encoding gene for the major outer membrane protein of Chlamydia trachomatis.

Authors:  F E Nano; P A Barstad; L W Mayer; J E Coligan; H D Caldwell
Journal:  Infect Immun       Date:  1985-05       Impact factor: 3.441

3.  Trypsin action on the growth of Sendai virus in tissue culture cells. I. Restoration of the infectivity for L cells by direct action of tyrpsin on L cell-borne Sendai virus.

Authors:  M Homma
Journal:  J Virol       Date:  1971-11       Impact factor: 5.103

4.  Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAE-dextran.

Authors:  C Kuo; S Wang; B B Wentworth; J T Grayston
Journal:  J Infect Dis       Date:  1972-06       Impact factor: 5.226

5.  Trypsin action on the growth of Sendai virus in tissue culture cells. IV. Evidence for activation of sendai virus by cleavage of a glycoprotein.

Authors:  M Ohuchi; M Homma
Journal:  J Virol       Date:  1976-06       Impact factor: 5.103

6.  A sensitive cell line, HL cells, for isolation and propagation of Chlamydia pneumoniae strain TWAR.

Authors:  C C Kuo; J T Grayston
Journal:  J Infect Dis       Date:  1990-09       Impact factor: 5.226

7.  Effect of polycations, polyanions and neuraminidase on the infectivity of trachoma-inclusin conjunctivitis and lymphogranuloma venereum organisms HeLa cells: sialic acid residues as possible receptors for trachoma-inclusion conjunction.

Authors:  C C Kuo; S P Wang; J T Grayston
Journal:  Infect Immun       Date:  1973-07       Impact factor: 3.441

8.  Pneumonia associated with the TWAR strain of Chlamydia.

Authors:  T J Marrie; J T Grayston; S P Wang; C C Kuo
Journal:  Ann Intern Med       Date:  1987-04       Impact factor: 25.391

Review 9.  Current knowledge on Chlamydia pneumoniae, strain TWAR, an important cause of pneumonia and other acute respiratory diseases.

Authors:  J T Grayston; S P Wang; C C Kuo; L A Campbell
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1989-03       Impact factor: 3.267

10.  [A simple method for differentiation of hydrogen sulfide-producing bacteria by the pH-dependent EDTA-sensitivity test].

Authors:  N Kida; M Matsuzaki; A Matsukawa; S Suzuki; N Ushiama; K Kawahara; F Taguchi
Journal:  Nihon Saikingaku Zasshi       Date:  1994-09
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  2 in total

1.  Optimizing culture of Chlamydia pneumoniae by using multiple centrifugations.

Authors:  J M Pruckler; N Masse; V A Stevens; L Gang; Y Yang; E R Zell; S F Dowell; B S Fields
Journal:  J Clin Microbiol       Date:  1999-10       Impact factor: 5.948

2.  Rapid detection of Chlamydia pneumoniae by PCR-enzyme immunoassay.

Authors:  C A Jantos; R Roggendorf; F N Wuppermann; J H Hegemann
Journal:  J Clin Microbiol       Date:  1998-07       Impact factor: 5.948
  2 in total

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