Literature DB >> 9163488

Rapid discrimination of Mycobacterium avium strains from AIDS patients by randomly amplified polymorphic DNA analysis.

P Matsiota-Bernard1, S Waser, P T Tassios, A Kyriakopoulos, N J Legakis.   

Abstract

A randomly amplified polymorphic DNA (RAPD) analysis was performed for the molecular typing of Mycobacterium avium strains. This method was applied to epidemiologically unrelated M. avium strains isolated from the blood of 10 different AIDS patients and to strains that were considered epidemiologically related, as they had been isolated from the same patient but from different body locations (4 patients, 10 strains). Three oligonucleotide primers among the six tested were found to generate RAPD profiles with DNA from all M. avium strains and to successfully type them. This method for the typing of M. avium strains is rapid and easy to perform.

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Year:  1997        PMID： 9163488      PMCID： PMC229793          DOI： 10.1128/jcm.35.6.1585-1588.1997

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

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3.  Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis.

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5.  Large DNA restriction fragment polymorphism in the Mycobacterium avium-M. intracellulare complex: a potential epidemiologic tool.

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7.  Clinical and epidemiological importance of typing of Mycobacterium avium complex isolates.

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Authors:  B C Ross; B Dwyer
Journal:  J Clin Microbiol       Date:  1993-02       Impact factor: 5.948

9.  Use of restriction fragment length polymorphism as a genetic marker for typing Mycobacterium avium strains.

Authors:  M P Roiz; E Palenque; C Guerrero; M J Garcia
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

10.  Incidence of Mycobacterium avium-intracellulare complex bacteremia in human immunodeficiency virus-positive patients.

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  3 in total

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2.  Molecular analysis of Mycobacterium avium isolates by using pulsed-field gel electrophoresis and PCR.

Authors:  M Pestel-Caron; G Graff; G Berthelot; J L Pons; J F Lemeland
Journal:  J Clin Microbiol       Date:  1999-08       Impact factor: 5.948

3.  Characterization of rpoB mutations in rifampin-resistant clinical Mycobacterium tuberculosis isolates from Greece.

Authors:  P Matsiota-Bernard; G Vrioni; E Marinis
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  3 in total

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