In vitro release of eosinophil chemotactic factor of anaphylaxis (ECF-A) from guinea pig skin.

An in vitro release of the eosinophil chemotactic factor of anaphylaxis (ECF-A) in anaphylactic reactions was studied using skin slices from actively sensitized guinea pigs. ECF-A and histamine were released in vitro from actively sensitized guinea pig skin by antigen challenge. The magnitude of ECF-A release did not always parallel that of anaphylactic histamine release in guinea pig skin, suggesting that there may be no correlation between the amount of ECF-A and that of histamine released from the skin in anaphylactic reactions in guinea pigs.