| Literature DB >> 9157676 |
G Entrican1, D Deane, M MacLean, L Inglis, J Thomson, C McInnes, D M Haig.
Abstract
Recombinant ovine granulocyte/macrophage colony-stimulating factor (rOv GM-CSF) has been expressed in Chinese hamster ovary cells. A stable, cloned line of these cells has been established which secretes high levels (40 mu g ml(-1)) of rOv GM-CSF. Three murine monoclonal antibodies (mAbs) were produced which reacted with rOv GM-CSF on Western blots. These mAbs also neutralised the activity of both recombinant and native Ov GM-CSF in a bone marrow haemopoietic progenitor cell assay. Two of the mAbs, which recognise mutually exclusive epitopes, were selected for the development of a sandwich enzyme-linked immunosorbent assay (ELISA) to measure GM-CSF in biological samples of ovine origin.Entities:
Mesh：
Substances：
Year: 1996 PMID： 9157676 DOI： 10.1016/0165-2427(95)05468-5
Source DB: PubMed Journal: Vet Immunol Immunopathol ISSN： 0165-2427 Impact factor: 2.046