Literature DB >> 9154992

Characterization of a structurally and functionally diverged acyl-acyl carrier protein desaturase from milkweed seed.

E B Cahoon1, S J Coughlan, J Shanklin.   

Abstract

A cDNA for a structurally variant acyl-acyl carrier protein (ACP) desaturase was isolated from milkweed (Asclepias syriaca) seed, a tissue enriched in palmitoleic (16:1delta9)* and cis-vaccenic (18:1delta11) acids. Extracts of Escherichia coli that express the milkweed cDNA catalyzed delta9 desaturation of acyl-ACP substrates, and the recombinant enzyme exhibited seven- to ten-fold greater specificity for palmitoyl (16:0)-ACP and 30-fold greater specificity for myristoyl (14:0)-ACP than did known delta9-stearoyl (18:0)-ACP desaturases. Like other variant acyl-ACP desaturases reported to date, the milkweed enzyme contains fewer amino acids near its N-terminus compared to previously characterized delta9-18:0-ACP desaturases. Based on the activity of an N-terminal deletion mutant of a delta9-18:0-ACP desaturase, this structural feature likely does not account for differences in substrate specificities.

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Year:  1997        PMID： 9154992     DOI： 10.1023/a:1005821007291

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  20 in total

1.  Development of oil in the seed of Asclepias syriaca L.

Authors:  C Y HOPKINS; M J CHISHOLM
Journal:  Can J Biochem Physiol       Date:  1961-05

Review 2.  Lipid biosynthesis.

Authors:  J Ohlrogge; J Browse
Journal:  Plant Cell       Date:  1995-07       Impact factor: 11.277

3.  Preparative enzymatic synthesis and hydrophobic chromatography of acyl-acyl carrier protein.

Authors:  C O Rock; J L Garwin
Journal:  J Biol Chem       Date:  1979-08-10       Impact factor: 5.157

4.  cDNAs for isoforms of the delta 9-stearoyl-acyl carrier protein desaturase from Thunbergia alata endosperm.

Authors:  E B Cahoon; C K Becker; J Shanklin; J B Ohlrogge
Journal:  Plant Physiol       Date:  1994-10       Impact factor: 8.340

5.  Expression of a delta 9 14:0-acyl carrier protein fatty acid desaturase gene is necessary for the production of omega 5 anacardic acids found in pest-resistant geranium (Pelargonium xhortorum).

Authors:  D J Schultz; E B Cahoon; J Shanklin; R Craig; D L Cox-Foster; R O Mumma; J I Medford
Journal:  Proc Natl Acad Sci U S A       Date:  1996-08-06       Impact factor: 11.205

6.  Primary structures of the precursor and mature forms of stearoyl-acyl carrier protein desaturase from safflower embryos and requirement of ferredoxin for enzyme activity.

Authors:  G A Thompson; D E Scherer; S Foxall-Van Aken; J W Kenny; H L Young; D K Shintani; J C Kridl; V C Knauf
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

7.  Stearoyl-acyl-carrier-protein desaturase from higher plants is structurally unrelated to the animal and fungal homologs.

Authors:  J Shanklin; C Somerville
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

8.  Metabolic Evidence for the Involvement of a [delta]4-Palmitoyl-Acyl Carrier Protein Desaturase in Petroselinic Acid Synthesis in Coriander Endosperm and Transgenic Tobacco Cells.

Authors:  E. B. Cahoon; J. B. Ohlrogge
Journal:  Plant Physiol       Date:  1994-03       Impact factor: 8.340

9.  delta 6 Hexadecenoic acid is synthesized by the activity of a soluble delta 6 palmitoyl-acyl carrier protein desaturase in Thunbergia alata endosperm.

Authors:  E B Cahoon; A M Cranmer; J Shanklin; J B Ohlrogge
Journal:  J Biol Chem       Date:  1994-11-04       Impact factor: 5.157

10.  Expression of a coriander desaturase results in petroselinic acid production in transgenic tobacco.

Authors:  E B Cahoon; J Shanklin; J B Ohlrogge
Journal:  Proc Natl Acad Sci U S A       Date:  1992-12-01       Impact factor: 11.205
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  12 in total

1.  Stearoyl-acyl carrier protein and unusual acyl-acyl carrier protein desaturase activities are differentially influenced by ferredoxin.

Authors:  D J Schultz; M C Suh; J B Ohlrogge
Journal:  Plant Physiol       Date:  2000-10       Impact factor: 8.340

2.  Substrate-dependent mutant complementation to select fatty acid desaturase variants for metabolic engineering of plant seed oils.

Authors:  E B Cahoon; J Shanklin
Journal:  Proc Natl Acad Sci U S A       Date:  2000-10-24       Impact factor: 11.205

3.  Oxygen-independent alkane formation by non-heme iron-dependent cyanobacterial aldehyde decarbonylase: investigation of kinetics and requirement for an external electron donor.

Authors:  Bekir E Eser; Debasis Das; Jaehong Han; Patrik R Jones; E Neil G Marsh
Journal:  Biochemistry       Date:  2011-11-15       Impact factor: 3.162

4.  A single mutation in the castor Delta9-18:0-desaturase changes reaction partitioning from desaturation to oxidase chemistry.

Authors:  Jodie E Guy; Isabel A Abreu; Martin Moche; Ylva Lindqvist; Edward Whittle; John Shanklin
Journal:  Proc Natl Acad Sci U S A       Date:  2006-11-06       Impact factor: 11.205

5.  Metabolic engineering of seeds can achieve levels of omega-7 fatty acids comparable with the highest levels found in natural plant sources.

Authors:  Huu Tam Nguyen; Girish Mishra; Edward Whittle; Mark S Pidkowich; Scott A Bevan; Ann Owens Merlo; Terence A Walsh; John Shanklin
Journal:  Plant Physiol       Date:  2010-10-13       Impact factor: 8.340

6.  A determinant of substrate specificity predicted from the acyl-acyl carrier protein desaturase of developing cat's claw seed.

Authors:  E B Cahoon; S Shah; J Shanklin; J Browse
Journal:  Plant Physiol       Date:  1998-06       Impact factor: 8.340

7.  ACYL-ACYL CARRIER PROTEIN DESATURASE2 and 3 Are Responsible for Making Omega-7 Fatty Acids in the Arabidopsis Aleurone.

Authors:  Fiona M Bryant; Olaya Munoz-Azcarate; Amélie A Kelly; Frédéric Beaudoin; Smita Kurup; Peter J Eastmond
Journal:  Plant Physiol       Date:  2016-07-26       Impact factor: 8.340

Review 8.  Synthetic redesign of plant lipid metabolism.

Authors:  Richard P Haslam; Olga Sayanova; Hae Jin Kim; Edgar B Cahoon; Johnathan A Napier
Journal:  Plant J       Date:  2016-06-20       Impact factor: 6.417

9.  Conserved Function of ACYL-ACYL CARRIER PROTEIN DESATURASE 5 on Seed Oil and Oleic Acid Biosynthesis between Arabidopsis thaliana and Brassica napus.

Authors:  Changyu Jin; Dong Li; Chenhao Gao; Kaige Liu; Shuanghui Qi; Shaowei Duan; Zixiong Li; Jingyun Gong; Jianjun Wang; Jiangbo Hai; Mingxun Chen
Journal:  Front Plant Sci       Date:  2017-07-25       Impact factor: 5.753

10.  Identification and functional analysis of delta-9 desaturase, a key enzyme in PUFA Synthesis, isolated from the oleaginous diatom Fistulifera.

Authors:  Masaki Muto; Chihiro Kubota; Masayoshi Tanaka; Akira Satoh; Mitsufumi Matsumoto; Tomoko Yoshino; Tsuyoshi Tanaka
Journal:  PLoS One       Date:  2013-09-05       Impact factor: 3.240
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