J Verran1, C J Maryan. 1. Department of Biological Sciences, Manchester Metropolitan University, United Kingdom.
Abstract
STATEMENT OF PROBLEM: The adhesion of microorganisms to a denture surface is a prerequisite for colonization. PURPOSE: This study compared the retention of Candida albicans on smooth and rough acrylic resin and silicone surfaces after a washing procedure to determine the effect of surface roughness on prosthesis infection and hygiene. MATERIAL AND METHODS: Standardized cell suspensions of C. albicans were incubated with smooth and rough acrylic resin and silicone surfaces for 1 hour at 24 degrees C. After washing, cells that had been retained on the surface were stained with acridine orange and examined with incident beam fluorescent microscopy. RESULTS: There was no significant difference in cell numbers on either of the smooth surfaces. Significantly higher numbers of cells (p > 0.0005) were observed on roughened surfaces (silicone > acrylic resin) than on smooth surfaces. The fitting surface of the maxillary denture was not polished. CONCLUSIONS: Silicones used in prostheses were processed against dental stone. The resultant surface roughness may facilitate microbial retention and infection and should therefore be kept to a minimum.
STATEMENT OF PROBLEM: The adhesion of microorganisms to a denture surface is a prerequisite for colonization. PURPOSE: This study compared the retention of Candida albicans on smooth and rough acrylic resin and silicone surfaces after a washing procedure to determine the effect of surface roughness on prosthesis infection and hygiene. MATERIAL AND METHODS: Standardized cell suspensions of C. albicans were incubated with smooth and rough acrylic resin and silicone surfaces for 1 hour at 24 degrees C. After washing, cells that had been retained on the surface were stained with acridine orange and examined with incident beam fluorescent microscopy. RESULTS: There was no significant difference in cell numbers on either of the smooth surfaces. Significantly higher numbers of cells (p > 0.0005) were observed on roughened surfaces (silicone > acrylic resin) than on smooth surfaces. The fitting surface of the maxillary denture was not polished. CONCLUSIONS:Silicones used in prostheses were processed against dental stone. The resultant surface roughness may facilitate microbial retention and infection and should therefore be kept to a minimum.