Literature DB >> 9149881

Optimization of cellular ELISA for assay of surface antigens on human synoviocytes.

D D Smith1, C B Cohick, H B Lindsley.   

Abstract

The cellular enzyme-linked immunosorbent assay (CELISA) permits assay of cell surface antigens on intact fixed cells. Using a monolayer of cells as the solid phase, the CELISA offers an inexpensive alternative to flow cytometry. In addition, this protocol has the decided advantages of miniaturization (small numbers of cells) and ease of replication (the 96-well cluster plate format). In efforts to optimize CELISA for detecting surface antigens on human fibroblastlike synoviocytes, the authors found that cell number, serum proteins, choice of culture plate, pipetting technique and fixatives may all impact the results of the CELISA.

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Year:  1997        PMID： 9149881      PMCID： PMC4453829          DOI： 10.2144/97225rr03

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  23 in total

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4.  Automaticity of social behavior: direct effects of trait construct and stereotype-activation on action.

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Review 8.  The uniqueness of speech among motor systems.

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Authors:  Francis S Collins; Lawrence A Tabak
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10.  Two failures to replicate high-performance-goal priming effects.

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  2 in total

1.  Development and validation of a cell-based fluorescent method for measuring antibody affinity.

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Journal:  J Immunol Methods       Date:  2016-12-24       Impact factor: 2.303

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  2 in total

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