Literature DB >> 9149806

Baculovirus cDNA libraries for expression cloning of genes encoding cell-surface antigens.

L Granziero1, P Nelboeck, M Bedoucha, A Lanzavecchia, H H Reid.   

Abstract

We describe a method for the production of baculovirus-based cDNA libraries. By staining with monoclonal antibodies, single positive cells can be sorted and the virus encoding for the surface epitope can be isolated by limiting dilution. We have used this method to isolate cDNAs encoding several cell-surface antigens.

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Year:  1997        PMID： 9149806     DOI： 10.1016/s0022-1759(97)00018-5

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  4 in total

1.  High-efficiency system for the construction of adenovirus vectors and its application to the generation of representative adenovirus-based cDNA expression libraries.

Authors:  Moritz Hillgenberg; Christian Hofmann; Herbert Stadler; Peter Löser
Journal:  J Virol       Date:  2006-06       Impact factor: 5.103

2.  A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo.

Authors:  Olli H Laitinen; Kari J Airenne; Vesa P Hytönen; Erik Peltomaa; Anssi J Mähönen; Thomas Wirth; Miia M Lind; Kari A Mäkelä; Pyry I Toivanen; Diana Schenkwein; Tommi Heikura; Henri R Nordlund; Markku S Kulomaa; Seppo Ylä-Herttuala
Journal:  Nucleic Acids Res       Date:  2005-02-24       Impact factor: 16.971

Review 3.  Baculovirus display: a multifunctional technology for gene delivery and eukaryotic library development.

Authors:  Anna R Mäkelä; Christian Oker-Blom
Journal:  Adv Virus Res       Date:  2006       Impact factor: 9.937

4.  Applications of cell sorting in biotechnology.

Authors:  Diethard Mattanovich; Nicole Borth
Journal:  Microb Cell Fact       Date:  2006-03-21       Impact factor: 5.328
  4 in total

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