Literature DB >> 9148756

The role of NAD+ as a signal during nitrogenase switch-off in Rhodospirillum rubrum.

A Norén1, A Soliman, S Nordlund.   

Abstract

The role of NAD+ in the metabolic regulation of nitrogenase, the 'switch-off' effect, in Rhodospirillum rubrum has been studied. We now show that the decrease in nitrogenase activity upon addition of NAD+ to R. rubrum is due to modification of dinitrogenase reductase. There was no effect when NAD+ was added to a mutant of R. rubrum devoid of dinitrogenase reductase ADP-ribosyltransferase, indicating that NAD+ 'switch-off' is an effect of the same regulatory system as ammonium 'switch-off'. We also show that oxaloacetate and alpha-ketoglutarate function as 'switch-off' effectors. On the other hand beta-hydroxybutyrate has the opposite effect by shortening the 'switch-off' period. Furthermore, by using an inhibitor of glutamate synthase the role of this enzyme in 'switch-off' was investigated. The results are discussed in relation to our proposal that changes in the concentration of NAD+ are involved in initiating 'switch-off'.

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Year:  1997        PMID： 9148756      PMCID： PMC1218262          DOI： 10.1042/bj3220829

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  21 in total

1.  A cold-sensitive D(-) beta-hydroxybutyric acid dehydrogenase from Rhodospirillum rubrum.

Authors:  C W SHUSTER; M DOUDOROFF
Journal:  J Biol Chem       Date:  1962-02       Impact factor: 5.157

2.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

3.  Energy-linked reduction of nicotinamide adenine dinucleotides in cells of Rhodospirillum rubrum.

Authors:  J B Jackson; A R Crofts
Journal:  Biochem Biophys Res Commun       Date:  1968-09-30       Impact factor: 3.575

4.  Effect of ammonia, darkness, and phenazine methosulfate on whole-cell nitrogenase activity and Fe protein modification in Rhodospirillum rubrum.

Authors:  R H Kanemoto; P W Ludden
Journal:  J Bacteriol       Date:  1984-05       Impact factor: 3.490

5.  Regulation of nitrogenase synthesis in intact cells of Rhodospirillum rubrum: inactivation of nitrogen fixation by ammonia, L-glutamine and L-asparagine.

Authors:  A H Neilson; S Nordlund
Journal:  J Gen Microbiol       Date:  1975-11

6.  Regulatory properties of the nitrogenase from Rhodopseudomonas palustris.

Authors:  W G Zumft; F Castillo
Journal:  Arch Microbiol       Date:  1978-04-27       Impact factor: 2.552

7.  Adenine nucleotide levels in Rhodospirillum rubrum during switch-off of whole-cell nitrogenase activity.

Authors:  T D Paul; P W Ludden
Journal:  Biochem J       Date:  1984-12-15       Impact factor: 3.857

8.  Covalent modification of the iron protein of nitrogenase from Rhodospirillum rubrum by adenosine diphosphoribosylation of a specific arginine residue.

Authors:  M R Pope; S A Murrell; P W Ludden
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

9.  Effect of light intensity and inhibitors of nitrogen assimilation on NH4+ inhibition of nitrogenase activity in Rhodospirillum rubrum and Anabaena sp.

Authors:  D C Yoch; J W Gotto
Journal:  J Bacteriol       Date:  1982-08       Impact factor: 3.490

10.  Changes in amino acid and nucleotide pools of Rhodospirillum rubrum during switch-off of nitrogenase activity initiated by NH4+ or darkness.

Authors:  J D Li; C Z Hu; D C Yoch
Journal:  J Bacteriol       Date:  1987-01       Impact factor: 3.490
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  6 in total

Review 1.  P(II) signal transduction proteins, pivotal players in microbial nitrogen control.

Authors:  T Arcondéguy; R Jack; M Merrick
Journal:  Microbiol Mol Biol Rev       Date:  2001-03       Impact factor: 11.056

2.  Dinitrogenase reductase-activating glycohydrolase can be released from chromatophores of Rhodospirillum rubrum by treatment with MgGDP.

Authors:  A Norén; S Nordlund
Journal:  J Bacteriol       Date:  1997-12       Impact factor: 3.490

3.  Functional characterization of three GlnB homologs in the photosynthetic bacterium Rhodospirillum rubrum: roles in sensing ammonium and energy status.

Authors:  Y Zhang; E L Pohlmann; P W Ludden; G P Roberts
Journal:  J Bacteriol       Date:  2001-11       Impact factor: 3.490

4.  Metabolic flux analysis of Cyanothece sp. ATCC 51142 under mixotrophic conditions.

Authors:  Swathi Alagesan; Sandeep B Gaudana; Avinash Sinha; Pramod P Wangikar
Journal:  Photosynth Res       Date:  2013-08-18       Impact factor: 3.573

5.  Effects of perturbations of the nitrogenase electron transfer chain on reversible ADP-ribosylation of nitrogenase Fe protein in Klebsiella pneumoniae strains bearing the Rhodospirillum rubrum dra operon.

Authors:  C M Halbleib; Y Zhang; G P Roberts; P W Ludden
Journal:  J Bacteriol       Date:  2000-07       Impact factor: 3.490

6.  Posttranslational modification of dinitrogenase reductase in Rhodospirillum rubrum treated with fluoroacetate.

Authors:  Natalia Akentieva
Journal:  World J Microbiol Biotechnol       Date:  2018-11-28       Impact factor: 3.312
  6 in total

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