Literature DB >> 914658

DNA-fluorometry of mammalian sperm.

J Zante, J Schumann, W Göhde, U Hacker.   

Abstract

The Dna-content of sperm and testicular cells was measured by pulse-cytophotometry with high resolution. From flat sperm symmetric and narrow fluorescene distributions were obtained. Enzymatic treatment with papain or pronase and staining with an ethidiumbromide-mithramycin dye solution generate stoichiometric DNA-staining including that of mature sperm with a coefficient of variation below 2%.

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Year:  1977        PMID： 914658     DOI： 10.1007/bf00493324

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  5 in total

1.  AUTORADIOGRAPHIC EVIDENCE OF A NUCLEAR HISTONE SYNTHESIS DURING MOUSE SPERMIOGENESIS IN THE ABSENCE OF DETECTABLE QUANTITIES OF NUCLEAR RIBONUCLEIC ACID.

Authors:  V MONESI
Journal:  Exp Cell Res       Date:  1964-12       Impact factor: 3.905

2.  [Flow fluorescence cytophotometry for ultra-rapid DNA measurements on large cell populations].

Authors:  E Sprenger; N Böhm; W Sandritter
Journal:  Histochemie       Date:  1971

3.  Changes in deoxyribonucleoprotein during spermiogenesis in the bull.

Authors:  B L Gledhill; M P Gledhill; R Rigler; N R Ringertz
Journal:  Exp Cell Res       Date:  1966-03       Impact factor: 3.905

4.  Synthetic activities during spermatogenesis in the mouse RNA and protein.

Authors:  V Monesi
Journal:  Exp Cell Res       Date:  1965-08       Impact factor: 3.905

5.  Flow microfluorometric analysis of sperm DNA content: effect of cell shape on the fluorescence distribution.

Authors:  B L Gledhill; S Lake; L L Steinmetz; J W Gray; P N Dean; M A Van Dilla
Journal:  J Cell Physiol       Date:  1976-03       Impact factor: 6.384
  5 in total
  5 in total

1.  Flow cytometry of human spermatozoa.

Authors:  F J Otto; U Hacker; J Zante; J Schumann; W Göhde; M L Meistrich
Journal:  Histochemistry       Date:  1979-07-11

2.  "Cytogenetic" studies of spermatids of mice carrying Cattanach's translocation by flow cytometry.

Authors:  M L Meistrich; W Göhde; R A White; J L Longtin
Journal:  Chromosoma       Date:  1979       Impact factor: 4.316

3.  Effects of alpha-tocopherol on cadmium-induced toxicity in rat testis and spermatogenesis.

Authors:  Hoe Saeng Yang; Dong Keun Han; Jung Ran Kim; Jae Chul Sim
Journal:  J Korean Med Sci       Date:  2006-06       Impact factor: 2.153

4.  DNA distribution of mast cell populations in growing rats.

Authors:  L Enerbäck; I Rundquist
Journal:  Histochemistry       Date:  1981

5.  X-ray induced DNA damage and repair in germ cells of PARP1(-/-) male mice.

Authors:  Paola Villani; Anna Maria Fresegna; Roberto Ranaldi; Patrizia Eleuteri; Lorena Paris; Francesca Pacchierotti; Eugenia Cordelli
Journal:  Int J Mol Sci       Date:  2013-09-04       Impact factor: 5.923
  5 in total

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