Literature DB >> 9142132

Gel microdrop technology for rapid isolation of rare and high producer cells.

J C Weaver1, P McGrath, S Adams.   

Abstract

Secreted proteins are a therapeutic cornerstone of the biotechnology industry, and numerous recombinant products, including human growth hormone, human erythropoietin and granulocyte-colony stimulating factor, are now widely prescribed. In addition to bioprocessing applications, GMD technology should benefit the emerging fields of cellular and gene therapy. The ability to assess rapidly and precisely the productivity of cells for ex vivo cell screening and expansion is a unique approach with numerous research and therapeutic uses.

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Year:  1997        PMID: 9142132     DOI: 10.1038/nm0597-583

Source DB:  PubMed          Journal:  Nat Med        ISSN: 1078-8956            Impact factor:   53.440


  11 in total

1.  A high-throughput automated platform for the development of manufacturing cell lines for protein therapeutics.

Authors:  Shuangping Shi; Russ G G Condon; Liang Deng; Jason Saunders; Finn Hung; Yung-Shyeng Tsao; Zhong Liu
Journal:  J Vis Exp       Date:  2011-09-22       Impact factor: 1.355

2.  The gel microdrop secretion assay: Identification of a low productivity subpopulation arising during the production of human antibody in CHO cells.

Authors:  L Hammill; J Welles; G R Carson
Journal:  Cytotechnology       Date:  2000-10       Impact factor: 2.058

3.  Generation of Murine Monoclonal Antibodies by Hybridoma Technology.

Authors:  Pamela Holzlöhner; Katja Hanack
Journal:  J Vis Exp       Date:  2017-01-02       Impact factor: 1.355

Review 4.  Current state and recent advances in biopharmaceutical production in Escherichia coli, yeasts and mammalian cells.

Authors:  Aleš Berlec; Borut Strukelj
Journal:  J Ind Microbiol Biotechnol       Date:  2013-02-06       Impact factor: 3.346

5.  Microfluidic chip-based single-cell cloning to accelerate biologic production timelines.

Authors:  Jonathan Diep; Huong Le; Kim Le; Ewelina Zasadzinska; Jasmine Tat; Pheng Yam; Ryan Zastrow; Natalia Gomez; Jennitte Stevens
Journal:  Biotechnol Prog       Date:  2021-08-10

6.  Going native: Direct high throughput screening of secreted full-length IgG antibodies against cell membrane proteins.

Authors:  Yongliang Fang; Thach H Chu; Margaret E Ackerman; Karl E Griswold
Journal:  MAbs       Date:  2017-09-21       Impact factor: 5.857

7.  Capture and display of antibodies secreted by hybridoma cells enables fluorescent on-cell screening.

Authors:  Rama Devudu Puligedda; Rashmi Sharma; Fetweh H Al-Saleem; Diana Kouiavskaia; Arul Balaji Velu; Chandana Devi Kattala; George C Prendergast; David R Lynch; Konstantin Chumakov; Scott K Dessain
Journal:  MAbs       Date:  2019-02-22       Impact factor: 5.857

8.  New cell line development for antibody-producing Chinese hamster ovary cells using split green fluorescent protein.

Authors:  Yeon-Gu Kim; Byoungwoo Park; Jung Oh Ahn; Joon-Ki Jung; Hong Weon Lee; Eun Gyo Lee
Journal:  BMC Biotechnol       Date:  2012-05-15       Impact factor: 2.563

9.  Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins.

Authors:  Antoine W Caron; Claire Nicolas; Bruno Gaillet; Ismaïla Ba; Maxime Pinard; Alain Garnier; Bernard Massie; Rénald Gilbert
Journal:  BMC Biotechnol       Date:  2009-05-11       Impact factor: 2.563

10.  Functionalizing cell-mimetic giant vesicles with encapsulated bacterial biosensors.

Authors:  Tatiana Trantidou; Linda Dekker; Karen Polizzi; Oscar Ces; Yuval Elani
Journal:  Interface Focus       Date:  2018-08-17       Impact factor: 3.906

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