E Suhler1, W Lin, H L Yin, W M Lee. 1. Liver Unit, University of Texas Southwestern Medical School, Dallas, USA.
Abstract
OBJECTIVE: To quantitate gelsolin concentrations in serum of patients with a variety of conditions involving actin release into the circulation. DESIGN: Prospective evaluation of sera on consecutive patients. SETTING: Metropolitan county hospital. PATIENTS: Ninety hospital patients with a variety of well-characterized diseases. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Sera were studied from patients with acute liver failure (n = 18), chronic hepatitis (n = 17), cirrhosis of varying etiology (n = 17), pancreatitis (n = 10), acute myocardial infarction (n = 10), myonecrosis due either to polymyositis or crush injuries (n = 12), and septic shock (n = 6); results were compared with sera from healthy individuals (n = 25). Gelsolin was quantified by Western blotting with monoclonal anti-gelsolin and laser densitometry. Significant reductions in mean gelsolin concentrations compared with healthy controls were observed in patients with acute liver failure (47%), myocardial infarction (69%), sepsis (51%), and myonecrosis (66%). An inverse correlation was observed between gelsolin concentration and severity of illness, as indicated by the magnitude of serum enzyme concentrations. CONCLUSIONS: Gelsolin depletion occurs in a variety of tissue injury syndromes. Depletion of actin-scavenger capacity in the presence of continued actin release may affect outcome in situations of severe organ damage.
OBJECTIVE: To quantitate gelsolin concentrations in serum of patients with a variety of conditions involving actin release into the circulation. DESIGN: Prospective evaluation of sera on consecutive patients. SETTING: Metropolitan county hospital. PATIENTS: Ninety hospital patients with a variety of well-characterized diseases. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Sera were studied from patients with acute liver failure (n = 18), chronic hepatitis (n = 17), cirrhosis of varying etiology (n = 17), pancreatitis (n = 10), acute myocardial infarction (n = 10), myonecrosis due either to polymyositis or crush injuries (n = 12), and septic shock (n = 6); results were compared with sera from healthy individuals (n = 25). Gelsolin was quantified by Western blotting with monoclonal anti-gelsolin and laser densitometry. Significant reductions in mean gelsolin concentrations compared with healthy controls were observed in patients with acute liver failure (47%), myocardial infarction (69%), sepsis (51%), and myonecrosis (66%). An inverse correlation was observed between gelsolin concentration and severity of illness, as indicated by the magnitude of serum enzyme concentrations. CONCLUSIONS:Gelsolin depletion occurs in a variety of tissue injury syndromes. Depletion of actin-scavenger capacity in the presence of continued actin release may affect outcome in situations of severe organ damage.
Authors: Michael Schnoor; Alexander García Ponce; Eduardo Vadillo; Rosana Pelayo; Jan Rossaint; Alexander Zarbock Journal: Cell Mol Life Sci Date: 2017-02-02 Impact factor: 9.261
Authors: John Audley; Emily F Gliniewicz; Kol A Zarember; Hanna S Hong; Gal Wald; Douglas B Kuhns; Elizabeth Kang; Harry L Malech; Anthony F Suffredini; Robert J Noveck; Mark J Dinubile; Susan L Levinson; Thomas P Stossel; John I Gallin Journal: Inflammation Date: 2020-09-04 Impact factor: 4.092
Authors: Janeen R Jordan; Ernest E Moore; Sagar S Damle; Phillip Eckels; Jeffrey L Johnson; Jonathan P Roach; Jasmina S Redzic; Kirk C Hansen; Anirban Banerjee Journal: J Surg Res Date: 2007-11 Impact factor: 2.192
Authors: Alina Kułakowska; Nicholas J Ciccarelli; Qi Wen; Barbara Mroczko; Wiesław Drozdowski; Maciej Szmitkowski; Paul A Janmey; Robert Bucki Journal: BMC Neurol Date: 2010-11-01 Impact factor: 2.474