Literature DB >> 91417

Direct immunofluorescence tests with counterstains for detection of Chlamydia psittaci in infected avian tissues.

J Tessler, S S Stone, L A Page.   

Abstract

Different methods of preparation and serological evaluation of rabbit globulins for use in fluorescent antibody conjugate and different methods of counterstaining with fluorescent antibody tests were evaluated for detection of Chlamydia psittaci in infected turkey tissues. The agar gel precipitin reaction was that chosen for testing and selecting antiserums to be used for fluorescein isothiocyanate conjugation. The fluorescent antibody staining was most pronounced with conjugate made from globulins precipitated with ammonium sulfate. A direct fluorescent antibody method with Evans blue counterstain correctly identified "coded" specimens of C. psittaci-infected and noninfected turkey air sacs. However, naphthalene black was superior to Evans blue as a counterstain when infected pericardial sacs were tested.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 91417      PMCID： PMC1319921     

Source DB:  PubMed          Journal:  Can J Comp Med        ISSN： 0008-4050


  13 in total

1.  Immunofluorescent studies with trachoma and related antigens.

Authors:  R L NICHOLS; D E McCOMB
Journal:  J Immunol       Date:  1962-10       Impact factor: 5.422

2.  A simple fractionation method for preparation of fluorescein-labeled gamma globulin.

Authors:  J L RIGGS; P C LOH; W C EVELAND
Journal:  Proc Soc Exp Biol Med       Date:  1960-12

3.  The isolation and identification of ornithosis infection in turkeys by tissue culture and immunocytochemical staining.

Authors:  P DONALDSON; D E DAVIS; J R WATKINS; S E SULKIN
Journal:  Am J Vet Res       Date:  1958-10       Impact factor: 1.156

4.  A simple chromatographic method for preparation of gamma globulin.

Authors:  H B LEVY; H A SOBER
Journal:  Proc Soc Exp Biol Med       Date:  1960-01

5.  Staining bacterial smears with fluorescent antibody. IV. Grouping streptococci with fluorescent antibody.

Authors:  M D MOODY; E C ELLIS; E L UPDYKE
Journal:  J Bacteriol       Date:  1958-05       Impact factor: 3.490

6.  Identification of streptococci in bacterial mixtures and clinical specimens with fluorescent antibody.

Authors:  S HALPEREN; P DONALDSON; S E SULKIN
Journal:  J Bacteriol       Date:  1958-08       Impact factor: 3.490

7.  Staining bacterial smears with fluorescent antibody. I. General methods for Malleomyces pseudomallei.

Authors:  M GOLDMAN; M D MOODY; B M THOMASON
Journal:  J Bacteriol       Date:  1956-09       Impact factor: 3.490

8.  An epornitic of fatal chlamydiosis (ornithosis) in South Carolina turkeys.

Authors:  L A Page; W T Derieux; R C Cutlip
Journal:  J Am Vet Med Assoc       Date:  1975-01-15       Impact factor: 1.936

9.  PREPARATION OF FLUORESCEIN ISOTHIOCYANATE-LABELED GAMMA-GLOBULIN BY DIALYSIS, GEL FILTRATION, AND IONEXCHANGE CHROMATOGRAPHY IN COMBINATION.

Authors:  R E DEDMON; A W HOLMES; F DEINHARDT
Journal:  J Bacteriol       Date:  1965-03       Impact factor: 3.490

10.  Staining toxoplasma gondii with fluorescein-labelled antibody. II. A new serologic test for antibodies to Toxoplasma based upon inhibition of specific staining.

Authors:  M GOLDMAN
Journal:  J Exp Med       Date:  1957-06-01       Impact factor: 14.307
View more
  2 in total

1.  Goat serums for fluorescent antibody conjugates to chlamydial antigens.

Authors:  J Tessler
Journal:  Can J Comp Med       Date:  1984-04

2.  Effects of cortisone, cytochalasin B and cycloheximide on strains of Chlamydia psittaci in cell cultures.

Authors:  J Tessler
Journal:  Can J Comp Med       Date:  1983-07
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.