| Literature DB >> 9140917 |
C Dupuy1, D Buzoni-Gatel, A Touze, P Le Cann, D Bout, P Coursaget.
Abstract
Recombinant human papillomavirus (HPV) type 16 L1 virus-like particles (VLPs) expressed in the baculovirus system were used to investigate the cellular immune response to human papillomavirus type 16. The cell-mediated immune response was evaluated through immunization of mice with HPV 16 L1 virus-like particles using a lymphoproliferation assay and cytokine production and cytometric analysis of lymphocyte subsets. A significant proliferative response was observed which was associated with secretion of both interferon-gamma and interleukin-2. FACS analysis of splenic lymphocytes revealed that CD8+ T-cells were increased in the immunized mice. These results demonstrate that HPV 16 L1 VLPs induce a T-cell response characterized by a Th1 profile and confirm that the HPV 16 VLP is a reasonable candidate for vaccine development.Entities:
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Year: 1997 PMID： 9140917 DOI： 10.1006/mpat.1996.0113
Source DB: PubMed Journal: Microb Pathog ISSN： 0882-4010 Impact factor: 3.738