Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.

Literature DB >> 9139923

Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.

Y Zhang¹, S V Taylor, H J Chiu, T P Begley.

Abstract

The characterization of a three-gene operon (the thiC operon) at 331 min, which is involved in thiamine biosynthesis in Bacillus subtilis, is described. The first gene in the operon is homologous to transcription activators in the lysR family. The second and third genes (thiK and thiC) have been subcloned and overexpressed in Escherichia coli. ThiK (30 kDa) catalyzes the phosphorylation of 4-methyl-5-(beta-hydroxyethyl)thiazole. ThiC (27 kDa) catalyzes the substitution of the pyrophosphate of 2-methyl-4-amino-5-hydroxymethylpyrimidine pyrophosphate by 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate to yield thiamine phosphate. Transcription of the thiC operon is not regulated by thiamine or 2-methyl-4-amino-5-hydroxymethylpyrimidine and is only slightly repressed by 4-methyl-5-(beta-hydroxyethyl)thiazole.

Entities: Chemical Species

Mesh：

Substances：

Year: 1997 PMID： 9139923 PMCID： PMC179069 DOI： 10.1128/jb.179.9.3030-3035.1997

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

17 in total

1. Basic local alignment search tool.

Authors: S F Altschul; W Gish; W Miller; E W Myers; D J Lipman
Journal: J Mol Biol Date: 1990-10-05 Impact factor: 5.469

2. Positive regulation of glutamate biosynthesis in Bacillus subtilis.

Authors: D E Bohannon; A L Sonenshein
Journal: J Bacteriol Date: 1989-09 Impact factor: 3.490

3. Factors affecting a cyanogen bromide-based assay of thiamin.

Authors: D T Wyatt; M Lee; R E Hillman
Journal: Clin Chem Date: 1989-11 Impact factor: 8.327

4. nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine.

Authors: K Maundrell
Journal: J Biol Chem Date: 1990-07-05 Impact factor: 5.157

5. A large family of bacterial activator proteins.

Authors: S Henikoff; G W Haughn; J M Calvo; J C Wallace
Journal: Proc Natl Acad Sci U S A Date: 1988-09 Impact factor: 11.205

6. Physical and mapping properties of distant linkages between genetic markers in transformation of Bacillus subtilis.

Authors: M S Kelly
Journal: Mol Gen Genet Date: 1967

7. Biosynthesis of thiamin. Different biosynthetic routes of the thiazole moiety of thiamin in aerobic organisms and anaerobic organisms.

Authors: K Tazuya; M Morisaki; K Yamada; H Kumaoka; K Saiki
Journal: Biochem Int Date: 1987-01

Review 8. The biosynthesis and degradation of thiamin (vitamin B1).

Authors: T P Begley
Journal: Nat Prod Rep Date: 1996-06 Impact factor: 13.423

9. Chromosomal insertions of Tn917 in Bacillus subtilis.

Authors: M A Vandeyar; S A Zahler
Journal: J Bacteriol Date: 1986-08 Impact factor: 3.490

10. Different biosynthetic pathways of the pyrimidine moiety of thiamin in procaryotes and eucaryotes.

Authors: K Yamada; M Morisaki; H Kumaoka
Journal: Biochim Biophys Acta Date: 1983-03-15

12 in total

1. Identification of a protein subset of the anthrax spore immunome in humans immunized with the anthrax vaccine adsorbed preparation.

Authors: Indira T Kudva; Robert W Griffin; Jeonifer M Garren; Stephen B Calderwood; Manohar John
Journal: Infect Immun Date: 2005-09 Impact factor: 3.441

2. New structural insights and molecular-modelling studies of 4-methyl-5-beta-hydroxyethylthiazole kinase from Pyrococcus horikoshii OT3 (PhThiK).

Authors: Jeyaraman Jeyakanthan; Subbiah Thamotharan; Devadasan Velmurugan; Vaijayanthimala Surya Narayna Rao; Shanthi Nagarajan; Akeo Shinkai; Seiki Kuramitsu; Shigeyuki Yokoyama
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Date: 2009-09-18

3. Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.

Authors: Ghislain Schyns; Sébastien Potot; Yi Geng; Teresa M Barbosa; Adriano Henriques; John B Perkins
Journal: J Bacteriol Date: 2005-12 Impact factor: 3.490

4. RNA expression analysis using an antisense Bacillus subtilis genome array.

Authors: J M Lee; S Zhang; S Saha; S Santa Anna; C Jiang; J Perkins
Journal: J Bacteriol Date: 2001-12 Impact factor: 3.490

5. Evidence for horizontally transferred genes involved in the biosynthesis of vitamin B(1), B(5), and B(7) in Heterodera glycines.

Authors: James P Craig; Sadia Bekal; Terry Niblack; Leslie Domier; Kris N Lambert
Journal: J Nematol Date: 2009-12 Impact factor: 1.402

6. The folate branch of the methionine biosynthesis pathway in Streptomyces lividans: disruption of the 5,10-methylenetetrahydrofolate reductase gene leads to methionine auxotrophy.

Authors: J Blanco; J J Coque; J F Martin
Journal: J Bacteriol Date: 1998-03 Impact factor: 3.490

7. Identification and characterization of an operon in Salmonella typhimurium involved in thiamine biosynthesis.

Authors: L A Petersen; D M Downs
Journal: J Bacteriol Date: 1997-08 Impact factor: 3.490

Review 8. The structural and biochemical foundations of thiamin biosynthesis.

Authors: Christopher T Jurgenson; Tadhg P Begley; Steven E Ealick
Journal: Annu Rev Biochem Date: 2009 Impact factor: 23.643

9. Molecular characterization of the thi3 gene involved in thiamine biosynthesis in Zea mays: cDNA sequence and enzymatic and structural properties of the recombinant bifunctional protein with 4-amino-5-hydroxymethyl-2-methylpyrimidine (phosphate) kinase and thiamine monophosphate synthase activities.

Authors: Maria Rapala-Kozik; Mariusz Olczak; Katarzyna Ostrowska; Agata Starosta; Andrzej Kozik
Journal: Biochem J Date: 2007-12-01 Impact factor: 3.857

10. The vitamin B1 metabolism of Staphylococcus aureus is controlled at enzymatic and transcriptional levels.

Authors: Ingrid B Müller; Bärbel Bergmann; Matthew R Groves; Isabel Couto; Leonard Amaral; Tadhg P Begley; Rolf D Walter; Carsten Wrenger
Journal: PLoS One Date: 2009-11-03 Impact factor: 3.240