Literature DB >> 9139376

Detection of respiratory syncytial virus from clinical specimens: comparison between reverse transcription polymerase chain reaction and tissue culture.

S Tantivanich1, K Suphanaranonda, K Balachanda, R Anderson.   

Abstract

RT-PCR was compared with tissue culture to detect RSV from nasopharyngeal aspirates. RT-PCR was more sensitive and specific than tissue culture method. RT-PCR has sensitivity and specificity of 100% and 97%, respectively. The results indicate that RT-PCR can be used for detection of RSV in clinical specimens.

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Year:  1995        PMID： 9139376

Source DB:  PubMed          Journal:  Southeast Asian J Trop Med Public Health        ISSN： 0125-1562            Impact factor:   0.267


  5 in total

1.  Comparison of a multiplex reverse transcription-PCR-enzyme hybridization assay with conventional viral culture and immunofluorescence techniques for the detection of seven viral respiratory pathogens.

Authors:  L Liolios; A Jenney; D Spelman; T Kotsimbos; M Catton; S Wesselingh
Journal:  J Clin Microbiol       Date:  2001-08       Impact factor: 5.948

2.  Detection of human respiratory syncytial virus in respiratory samples by LightCycler reverse transcriptase PCR.

Authors:  David M Whiley; Melanie W Syrmis; Ian M Mackay; Theo P Sloots
Journal:  J Clin Microbiol       Date:  2002-12       Impact factor: 5.948

3.  Molecular epidemiology of respiratory viruses among Malaysian Young children with a confirmed respiratory infection during 2014-2015.

Authors:  Su Mei Yew; Ka-Liong Tan; Siok Koon Yeo; Kee Peng Ng; Chee Sian Kuan
Journal:  J Thorac Dis       Date:  2019-11       Impact factor: 2.895

4.  Detection of Respiratory Viruses from ARTI Patients by xTAG RVP Fast v2 Assay and Conventional Methods.

Authors:  Chee Sian Kuan; Su Mei Yew; Poh Sim Hooi; Lu Mei Lee; Kee Peng Ng
Journal:  Malays J Med Sci       Date:  2017-10-26

5.  Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays.

Authors:  M T Coiras; J C Aguilar; M L García; I Casas; P Pérez-Breña
Journal:  J Med Virol       Date:  2004-03       Impact factor: 2.327
  5 in total

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