Characterization of an interleukin 1 receptor antagonist protein released from ultraviolet-irradiated epidermal sheet.

An interleukin 1 (IL-1) inhibitor was detected in the culture supernatant of mouse epidermal sheet which had been irradiated with middle-wavelength ultraviolet (UVB) either in vitro or in vivo. Properties of the IL-1 inhibitor were consistent with those of IL-1 receptor antagonist (IL-1ra), i.e., approximate molecular size of 17 kDa by gel filtration, specific inhibition of IL-1-induced thymocyte proliferation, inhibition of binding of IL-1 to IL-1 receptor on the T-cell surface, and reactivity to anti-mouse IL-1ra antibody shown by immunoblotting and enzyme-linked immunosorbent assay (ELISA). These results indicate that this IL-1 inhibitor, which appears to be identical with IL-1ra, was released from the epidermis upon UVB irradiation, and that the inhibitor may participate in the regulation of inflammation mediated by IL-1, as well as in the pathogenesis of ultraviolet (UV)-induced immunosuppression.