Evaluation of the Phene Plate generalized microplate for metabolic fingerprinting and for measuring fermentative capacity of mixed bacterial populations.

The Phene Plate (PhP) generalized microplate for metabolic fingerprinting and for measuring the fermentative capacity of intestinal bacteria was evaluated. Twelve bacterial species, representing those commonly found in the intestine of humans and animals were employed. Mixtures of bacteria were inoculated in duplicate onto the PhP microplates. Anaerobic conditions were achieved by either incubating the plates under nitrogen atmosphere or by covering the microplates with mineral oil before incubation. Different metabolic fingerprints based on the pattern of substrate utilization were obtained for each bacterial mixture. Metabolic responses of bacterial samples were similar under both anaerobic conditions although the rate of carbohydrate utilization was higher in plates covered with mineral oil. A fermentative capacity value based on the number and the degree of fermented carbohydrates was established for each mixture which differed as the composition of the mixture changed but in general it was higher in samples with more bacterial species. The PhP generalized microplate may thus be used for studying the functional status and metabolic potential of intestinal floras.