Literature DB >> 9133594

Insertionally inactivated and inducible recA alleles for use in Neisseria.

H S Seifert1.   

Abstract

Two classes of recA mutations have been constructed for use in Neisseria gonorrhoeae: three insertionally inactivated ('knockout') mutations and three LacI-regulatable constructs that can be shifted between Rec- and Rec+ by the removal or addition of IPTG. The effects of regulating recA expression on the processes of DNA transformation, DNA repair and pilin-phase variation are described. These regulatable cassettes can also be used to control the expression of any chromosomal gene.

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Year:  1997        PMID： 9133594     DOI： 10.1016/s0378-1119(96)00810-4

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  63 in total

1.  Insertion mutations in pilE differentially alter gonococcal pilin antigenic variation.

Authors:  B Howell-Adams; H S Seifert
Journal:  J Bacteriol       Date:  1999-10       Impact factor: 3.490

2.  Components and dynamics of fiber formation define a ubiquitous biogenesis pathway for bacterial pili.

Authors:  M Wolfgang; J P van Putten; S F Hayes; D Dorward; M Koomey
Journal:  EMBO J       Date:  2000-12-01       Impact factor: 11.598

3.  Roles of the recJ and recN genes in homologous recombination and DNA repair pathways of Neisseria gonorrhoeae.

Authors:  Eric P Skaar; Matthew P Lazio; H Steven Seifert
Journal:  J Bacteriol       Date:  2002-02       Impact factor: 3.490

4.  A mutant form of the Neisseria gonorrhoeae pilus secretin protein PilQ allows increased entry of heme and antimicrobial compounds.

Authors:  Ching-ju Chen; Deborah M Tobiason; Christopher E Thomas; William M Shafer; H Steven Seifert; P Frederick Sparling
Journal:  J Bacteriol       Date:  2004-02       Impact factor: 3.490

5.  Type IV pilus retraction in pathogenic Neisseria is regulated by the PilC proteins.

Authors:  Philippe C Morand; Emmanuelle Bille; Sandrine Morelle; Emmanuel Eugène; Jean-Luc Beretti; Matthew Wolfgang; Thomas F Meyer; Michael Koomey; Xavier Nassif
Journal:  EMBO J       Date:  2004-04-22       Impact factor: 11.598

6.  Genetic Manipulation of Neisseria gonorrhoeae.

Authors:  Joseph P Dillard
Journal:  Curr Protoc Microbiol       Date:  2011-11

7.  New complementation constructs for inducible and constitutive gene expression in Neisseria gonorrhoeae and Neisseria meningitidis.

Authors:  Meghan E Ramsey; Kathleen T Hackett; Chaitra Kotha; Joseph P Dillard
Journal:  Appl Environ Microbiol       Date:  2012-02-10       Impact factor: 4.792

8.  XerCD-mediated site-specific recombination leads to loss of the 57-kilobase gonococcal genetic island.

Authors:  Nadia M Domínguez; Kathleen T Hackett; Joseph P Dillard
Journal:  J Bacteriol       Date:  2010-11-12       Impact factor: 3.490

9.  Identification of ZipA, a signal recognition particle-dependent protein from Neisseria gonorrhoeae.

Authors:  Ying Du; Cindy Grove Arvidson
Journal:  J Bacteriol       Date:  2003-04       Impact factor: 3.490

10.  Resistance of Neisseria gonorrhoeae to non-oxidative killing by adherent human polymorphonuclear leucocytes.

Authors:  Alison K Criss; Ben Z Katz; H Steven Seifert
Journal:  Cell Microbiol       Date:  2009-03-12       Impact factor: 3.715
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