Literature DB >> 9131788

Cloning of sucrase operon with mini-Mu and plasmid-mediated metabolism of sucrose.

J Grones1, M Macor, V Bilská.   

Abstract

The in vivo cloning system based on mini-Mu derivatives was used for cloning the sucrase operon. We constructed several recombinant plasmids pJT21, pMM2324, pMM2325 with a complete sucrase operon. Two strains of Escherichia coli containing this plasmid replicon were able to grow on different concentrations of sucrose. The stability of recombinant plasmids was determined after cultivation under nonselective conditions. The stability after 5-d cultivation was higher than 75%.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 9131788     DOI： 10.1007/bf02814707

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  20 in total

1.  Characterization of transmissible genetic elements from sucrose-fermenting Salmonella strains.

Authors:  J A Wohlhieter; J R Lazere; N J Snellings; E M Johnson; R M Synenki; L S Baron
Journal:  J Bacteriol       Date:  1975-05       Impact factor: 3.490

2.  Instantaneous gene transfer from donor to recipient microorganisms via electroporation.

Authors:  J J Kilbane; B A Bielaga
Journal:  Biotechniques       Date:  1991-03       Impact factor: 1.993

3.  Nucleotide sequence of the sucrase gene of Bacillus subtilis.

Authors:  A Fouet; A Klier; G Rapoport
Journal:  Gene       Date:  1986       Impact factor: 3.688

4.  Transmissible substrate-utilizing ability in enterobacteria.

Authors:  H W Smith; Z Parsell
Journal:  J Gen Microbiol       Date:  1975-03

5.  MudSacI, a transposon with strong selectable and counterselectable markers: use for rapid mapping of chromosomal mutations in Salmonella typhimurium.

Authors:  M Lawes; S Maloy
Journal:  J Bacteriol       Date:  1995-03       Impact factor: 3.490

6.  Phosphoenolpyruvate-dependent phosphotransferase system enzyme III and plasmid-encoded sucrose transport in Escherichia coli K-12.

Authors:  J W Lengeler; R J Mayer; K Schmid
Journal:  J Bacteriol       Date:  1982-07       Impact factor: 3.490

7.  Mini-mu bacteriophage with plasmid replicons for in vivo cloning and lac gene fusing.

Authors:  E A Groisman; M J Casadaban
Journal:  J Bacteriol       Date:  1986-10       Impact factor: 3.490

8.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

9.  Molecular cloning and characterization of scrB, the structural gene for the Streptococcus mutans phosphoenolpyruvate-dependent sucrose phosphotransferase system sucrose-6-phosphate hydrolase.

Authors:  R D Lunsford; F L Macrina
Journal:  J Bacteriol       Date:  1986-05       Impact factor: 3.490

10.  Purification and properties of glucosyltransferase responsible for water-insoluble glucan synthesis from Streptococcus mutans.

Authors:  K Fukui; T Moriyama; Y Miyake; K Mizutani; O Tanaka
Journal:  Infect Immun       Date:  1982-07       Impact factor: 3.441
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.