Literature DB >> 9131489

In vitro techniques for studying drug metabolism.

F P Guengerich1.   

Abstract

A considerable amount of information has been obtained about human enzymes involved in drug metabolism. Cytochrome P450 is presented as a paradigm in order to illustrate the experimental techniques now available. In vitro assays can be done with tissue slices, microsomes, or even short-term cell cultures. In addition, recombinant enzymes can be produced in a variety of different vector systems. Information about catalytic selectivity regarding new drugs can be obtained, as well as important information about potential drug-drug interactions and bioavailability. Such studies play a role in the drug development process as well as metabolism and safety assessment.

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Year:  1996        PMID： 9131489     DOI： 10.1007/bf02353478

Source DB:  PubMed          Journal:  J Pharmacokinet Biopharm        ISSN： 0090-466X


  24 in total

1.  Interaction of citrus juices with felodipine and nifedipine.

Authors:  D G Bailey; J D Spence; C Munoz; J M Arnold
Journal:  Lancet       Date:  1991-02-02       Impact factor: 79.321

2.  In vitro inhibition of dihydropyridine oxidation and aflatoxin B1 activation in human liver microsomes by naringenin and other flavonoids.

Authors:  F P Guengerich; D H Kim
Journal:  Carcinogenesis       Date:  1990-12       Impact factor: 4.944

3.  Expression of mammalian cytochrome P450 enzymes using yeast-based vectors.

Authors:  F P Guengerich; W R Brian; M A Sari; J T Ross
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

Review 4.  Oxidation of toxic and carcinogenic chemicals by human cytochrome P-450 enzymes.

Authors:  F P Guengerich; T Shimada
Journal:  Chem Res Toxicol       Date:  1991 Jul-Aug       Impact factor: 3.739

Review 5.  Use of human hepatocyte cultures for drug metabolism studies.

Authors:  A Guillouzo; F Morel; O Fardel; B Meunier
Journal:  Toxicology       Date:  1993-10-05       Impact factor: 4.221

Review 6.  Recombinant yeast in drug metabolism.

Authors:  J P Renaud; M A Peyronneau; P Urban; G Truan; C Cullin; D Pompon; P Beaune; D Mansuy
Journal:  Toxicology       Date:  1993-10-05       Impact factor: 4.221

7.  In vitro metabolism of methylene chloride in human and animal tissues: use in physiologically based pharmacokinetic models.

Authors:  R H Reitz; A L Mendrala; F P Guengerich
Journal:  Toxicol Appl Pharmacol       Date:  1989-02       Impact factor: 4.219

8.  Expression of mammalian glutathione S-transferase 5-5 in Salmonella typhimurium TA1535 leads to base-pair mutations upon exposure to dihalomethanes.

Authors:  R Thier; J B Taylor; S E Pemble; W G Humphreys; M Persmark; B Ketterer; F P Guengerich
Journal:  Proc Natl Acad Sci U S A       Date:  1993-09-15       Impact factor: 11.205

9.  Physiologically based pharmacokinetics and the risk assessment process for methylene chloride.

Authors:  M E Andersen; H J Clewell; M L Gargas; F A Smith; R H Reitz
Journal:  Toxicol Appl Pharmacol       Date:  1987-02       Impact factor: 4.219

10.  Comparison of monooxygenase activities and cytochrome P-450 isozyme concentrations in human liver microsomes.

Authors:  P H Beaune; P G Kremers; L S Kaminsky; J De Graeve; A Albert; F P Guengerich
Journal:  Drug Metab Dispos       Date:  1986 Jul-Aug       Impact factor: 3.922
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  6 in total

Review 1.  Cytochrome P4503A (CYP3A) metabolism: prediction of in vivo activity in humans.

Authors:  G R Wilkinson
Journal:  J Pharmacokinet Biopharm       Date:  1996-10

2.  Melperone but not bisoprolol or metoprolol is a clinically relevant inhibitor of CYP2D6: evidence from a therapeutic drug monitoring survey.

Authors:  Gudrun Hefner; Stefan Unterecker; Mohamed E E Shams; Margarete Wolf; Tanja Falter; Ekkehard Haen; Christoph Hiemke
Journal:  J Neural Transm (Vienna)       Date:  2015-05-05       Impact factor: 3.575

3.  Effect of the flavonoids biochanin A and silymarin on the P-glycoprotein-mediated transport of digoxin and vinblastine in human intestinal Caco-2 cells.

Authors:  Shuzhong Zhang; Marilyn E Morris
Journal:  Pharm Res       Date:  2003-08       Impact factor: 4.200

Review 4.  Mulberry ( sang shèn zǐ) and its bioactive compounds, the chemoprevention effects and molecular mechanisms in vitro and in vivo.

Authors:  Hui-Pei Huang; Ting-Tsz Ou; Chau-Jong Wang
Journal:  J Tradit Complement Med       Date:  2013-01

5.  Traditional Medicine to Modern Pharmacogenomics: Ayurveda Prakriti Type and CYP2C19 Gene Polymorphism Associated with the Metabolic Variability.

Authors:  Yogita Ghodke; Kalpana Joshi; Bhushan Patwardhan
Journal:  Evid Based Complement Alternat Med       Date:  2011-06-08       Impact factor: 2.629

6.  Cell-based assay for the detection of chemically induced cellular stress by immortalized untransformed transgenic hepatocytes.

Authors:  Maria Grazia Sacco; Laura Amicone; Enrica Mira Catò; Daniela Filippini; Paolo Vezzoni; Marco Tripodi
Journal:  BMC Biotechnol       Date:  2004-03-19       Impact factor: 2.563
  6 in total

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