BACKGROUND: Manganese superoxide dismutase (MnSOD) from Aspergillus fumigatus has been demonstrated to be an allergen showing a high degree of homology with phylogenetically distant MnSODs. We describe cloning, production and characterization of MnSODs from different species. METHODS: MnSODs were cloned by PCR, expressed as inclusion body protein in Escherichia coli, purified by Ni2+-chelate affinity chromatography and characterized. RESULTS: The MnSODs from A. fumigatus, man, yeast, Drosophila melanogaster and E. coli show about 50% identity and 70% similarity on the primary structure. All proteins were produced at a high level in E. coli and refolded to achieve enzymatic activity. The proteins were able to bind IgE from sera of individuals sensitized to the A. fumigatus MnSOD. CONCLUSIONS: MnSOD represents a novel pan-allergen restricted to individuals sensitized to A. fumigatus.
BACKGROUND: Manganese superoxide dismutase (MnSOD) from Aspergillus fumigatus has been demonstrated to be an allergen showing a high degree of homology with phylogenetically distant MnSODs. We describe cloning, production and characterization of MnSODs from different species. METHODS: MnSODs were cloned by PCR, expressed as inclusion body protein in Escherichia coli, purified by Ni2+-chelate affinity chromatography and characterized. RESULTS: The MnSODs from A. fumigatus, man, yeast, Drosophila melanogaster and E. coli show about 50% identity and 70% similarity on the primary structure. All proteins were produced at a high level in E. coli and refolded to achieve enzymatic activity. The proteins were able to bind IgE from sera of individuals sensitized to the A. fumigatus MnSOD. CONCLUSIONS: MnSOD represents a novel pan-allergen restricted to individuals sensitized to A. fumigatus.
Authors: Ilona Kleine Budde; Pleuni G de Heer; Susanne Natter; Vera Mahler; Jaring S van der Zee; Rudolf Valenta; Rob C Aalberse Journal: Immunology Date: 2002-10 Impact factor: 7.397
Authors: C Mayer; U Appenzeller; H Seelbach; G Achatz; H Oberkofler; M Breitenbach; K Blaser; R Crameri Journal: J Exp Med Date: 1999-05-03 Impact factor: 14.307