Literature DB >> 912755

Detection of myosin heavy chain mRNA during myogenesis in tissue culture by in vitro and in situ hybridization.

H A John, M Patrinou-Georgoulas, K W Jones.   

Abstract

A cDNA copy of purified chick embryonic skeletal myosin heavy chain mRNA (MHC mRNA) distinguished between myogenic and nonmyogenic cells compared by in vitro and in situ hybridization. The majority of cells in replicating mononucleate myogenic cell cultures contained no detectable MHC mRNA. Among the earliest cells to contain MHC mRNA were cells engaged in mitosis. A relatively large amount of MHC mRNA was found in postmitotic monucleate cells and myotubes, and we observed nucleolar localization of MHC mRNA in these cells.

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Year:  1977        PMID： 912755     DOI： 10.1016/0092-8674(77)90126-x

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  18 in total

1.  Redistribution of myosin heavy chain mRNA in the midregion of stretched muscle fibers.

Authors:  D J Dix; B R Eisenberg
Journal:  Cell Tissue Res       Date:  1991-01       Impact factor: 5.249

2.  MicroRNAs with a nucleolar location.

Authors:  Joan C Ritland Politz; Eric M Hogan; Thoru Pederson
Journal:  RNA       Date:  2009-07-23       Impact factor: 4.942

3.  Regulation of tropomyosin gene expression during myogenesis.

Authors:  M Moss; R Schwartz
Journal:  Mol Cell Biol       Date:  1981-03       Impact factor: 4.272

4.  Most myosin heavy chain mRNA in L6E9 rat myotubes has a short poly(A) tail.

Authors:  S Benoff; B Nadal-Ginard
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

5.  Detection of AKR MuLV-specific RNA in AKR mouse cells by in situ hybridization.

Authors:  C Godard; K W Jones
Journal:  Nucleic Acids Res       Date:  1979-06-25       Impact factor: 16.971

6.  Quantitation of muscle-specific mRNAs by using cDNA probes during chicken embryonic muscle development in ovo.

Authors:  C R Saidapet; H N Munro; K Valgeirsdóttir; S Sarkar
Journal:  Proc Natl Acad Sci U S A       Date:  1982-05       Impact factor: 11.205

7.  Molecular cloning of developmentally regulated, low-abundance mRNA sequences from embryonic muscle.

Authors:  C P Ordahl; D Kioussis; S M Tilghman; C E Ovitt; J Fornwald
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205

8.  Improved method for detection of cellular transcripts by in situ hybridization: detection of poly (A) sequences in individual cells.

Authors:  C M Godard; K W Jones
Journal:  Histochemistry       Date:  1980

9.  Adaptation of a non-radioactive in situ hybridization method to electron microscopy: detection of tenascin mRNAs in mouse cerebellum with digoxigenin-labelled probes and gold-labelled antibodies.

Authors:  U Dörries; U Bartsch; C Nolte; J Roth; M Schachner
Journal:  Histochemistry       Date:  1993-03

10.  Detection of poly A+ RNA in sea urchin eggs and embryos by quantitative in situ hybridization.

Authors:  L M Angerer; R C Angerer
Journal:  Nucleic Acids Res       Date:  1981-06-25       Impact factor: 16.971
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