PURPOSE: A new polymerase chain reaction (PCR) test (Amplicor, Diagnostics, Branchburg, NJ) was evaluated for its ability to detect chlamydial DNA from previously obtained adult conjunctival specimens. METHODS: The sensitivity of this PCR test was determined on 42 adult conjunctival specimens that were culture-positive for Chlamydia. The specificity was determined by testing 40 true-negative specimens that included 10 normal conjunctival samples and 20 ocular specimens that were culture-positive for herpes simplex virus or adenovirus. The remaining ten samples consisted of ocular bacterial pathogens in chlamydial transport media. RESULTS: Amplicor was 88% (37/42) sensitive and 100% (40/40) specific. CONCLUSIONS: The authors conclude that PCR testing for chlamydial DNA from ocular specimens may be useful, especially when conditions in transport might reduce the yield of positive cultures.
PURPOSE: A new polymerase chain reaction (PCR) test (Amplicor, Diagnostics, Branchburg, NJ) was evaluated for its ability to detect chlamydial DNA from previously obtained adult conjunctival specimens. METHODS: The sensitivity of this PCR test was determined on 42 adult conjunctival specimens that were culture-positive for Chlamydia. The specificity was determined by testing 40 true-negative specimens that included 10 normal conjunctival samples and 20 ocular specimens that were culture-positive for herpes simplex virus or adenovirus. The remaining ten samples consisted of ocular bacterial pathogens in chlamydial transport media. RESULTS: Amplicor was 88% (37/42) sensitive and 100% (40/40) specific. CONCLUSIONS: The authors conclude that PCR testing for chlamydial DNA from ocular specimens may be useful, especially when conditions in transport might reduce the yield of positive cultures.
Authors: P Goldschmidt; H Rostane; C Saint-Jean; L Batellier; C Alouch; E Zito; T Bourcier; L Laroche; C Chaumeil Journal: Br J Ophthalmol Date: 2006-08-09 Impact factor: 4.638
Authors: Emma M Harding-Esch; Martin J Holland; Jean-François Schémann; Sandra Molina; Isatou Sarr; Aura A Andreasen; Chrissy h Roberts; Ansumana Sillah; Boubacar Sarr; Edward F Harding; Tansy Edwards; Robin L Bailey; David C W Mabey Journal: PLoS Negl Trop Dis Date: 2011-08-02