Literature DB >> 9119737

Effect of glutathione depletion on cisplatin resistance in cancer cells transfected with the gamma-glutamylcysteine synthetase gene.

H Kurokawa1, K Nishio, T Ishida, H Arioka, K Fukuoka, T Nomoto, H Fukumoto, H Yokote, N Saijo.   

Abstract

Overexpression of the human gamma-glutamylcysteine (gamma-GCS) gene resulted in cisplatin resistance with an increased glutathione (GSH) content, increased ATP-dependent glutathione S-conjugate export pump (GS-X pump) activity and decreased platinum accumulation in human lung cancer cells transfected with a gamma-GCS cDNA expression vector, as we previously reported. In this study, we examined the effects of buthionine sulfoximine (BSO), a specific inhibitor of gamma-GCS, to determine whether GSH depletion alters cisplatin resistance in a gamma-GCS-transfected cell line, SBC-3/GCS. In the presence of 10 microM BSO for 4 days, SBC-3/GCS still showed resistance to cisplatin, although it was partially reversed. Under these conditions, GS-X pump activity remained up-regulated in spite of low GSH content, and the platinum content was decreased. These data suggest that the GS-X pump itself influences cisplatin resistance, as well as cellular GSH content.

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Year:  1997        PMID： 9119737      PMCID： PMC5921367          DOI： 10.1111/j.1349-7006.1997.tb00354.x

Source DB:  PubMed          Journal:  Jpn J Cancer Res        ISSN： 0910-5050


  16 in total

1.  Coordinated induction of MRP/GS-X pump and gamma-glutamylcysteine synthetase by heavy metals in human leukemia cells.

Authors:  T Ishikawa; J J Bao; Y Yamane; K Akimaru; K Frindrich; C D Wright; M T Kuo
Journal:  J Biol Chem       Date:  1996-06-21       Impact factor: 5.157

2.  GS-X pump is functionally overexpressed in cis-diamminedichloroplatinum (II)-resistant human leukemia HL-60 cells and down-regulated by cell differentiation.

Authors:  T Ishikawa; C D Wright; H Ishizuka
Journal:  J Biol Chem       Date:  1994-11-18       Impact factor: 5.157

3.  Adenosine triphosphate-dependent transport of leukotriene C4 by membrane vesicles prepared from cisplatin-resistant human epidermoid carcinoma tumor cells.

Authors:  R Fujii; M Mutoh; T Sumizawa; Z S Chen; A Yoshimura; S Akiyama
Journal:  J Natl Cancer Inst       Date:  1994-12-07       Impact factor: 13.506

4.  Role of glutathione in the export of compounds from cells by the multidrug-resistance-associated protein.

Authors:  G J Zaman; J Lankelma; O van Tellingen; J Beijnen; H Dekker; C Paulusma; R P Oude Elferink; F Baas; P Borst
Journal:  Proc Natl Acad Sci U S A       Date:  1995-08-15       Impact factor: 11.205

5.  Overexpression of the gene encoding the multidrug resistance-associated protein results in increased ATP-dependent glutathione S-conjugate transport.

Authors:  M Müller; C Meijer; G J Zaman; P Borst; R J Scheper; N H Mulder; E G de Vries; P L Jansen
Journal:  Proc Natl Acad Sci U S A       Date:  1994-12-20       Impact factor: 11.205

6.  Multidrug resistance protein (MRP)-mediated transport of leukotriene C4 and chemotherapeutic agents in membrane vesicles. Demonstration of glutathione-dependent vincristine transport.

Authors:  D W Loe; K C Almquist; R G Deeley; S P Cole
Journal:  J Biol Chem       Date:  1996-04-19       Impact factor: 5.157

7.  Congenital jaundice in rats with a mutation in a multidrug resistance-associated protein gene.

Authors:  C C Paulusma; P J Bosma; G J Zaman; C T Bakker; M Otter; G L Scheffer; R J Scheper; P Borst; R P Oude Elferink
Journal:  Science       Date:  1996-02-23       Impact factor: 47.728

8.  Evidence for altered regulation of gamma-glutamylcysteine synthetase gene expression among cisplatin-sensitive and cisplatin-resistant human ovarian cancer cell lines.

Authors:  K S Yao; A K Godwin; S W Johnson; R F Ozols; P J O'Dwyer; T C Hamilton
Journal:  Cancer Res       Date:  1995-10-01       Impact factor: 12.701

9.  Gamma-glutamylcysteine synthetase gene overexpression results in increased activity of the ATP-dependent glutathione S-conjugate export pump and cisplatin resistance.

Authors:  H Kurokawa; T Ishida; K Nishio; H Arioka; M Sata; H Fukumoto; M Miura; N Saijo
Journal:  Biochem Biophys Res Commun       Date:  1995-11-02       Impact factor: 3.575

10.  Determinants of drug response in a cisplatin-resistant human lung cancer cell line.

Authors:  Y Fujiwara; Y Sugimoto; K Kasahara; M Bungo; M Yamakido; K D Tew; N Saijo
Journal:  Jpn J Cancer Res       Date:  1990-05
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  2 in total

1.  Glutathione content is correlated with the sensitivity of lines of PC12 cells to cisplatin without a corresponding change in the accumulation of platinum.

Authors:  K Ikeda; K Miura; S Himeno; N Imura; A Naganuma
Journal:  Mol Cell Biochem       Date:  2001-03       Impact factor: 3.396

2.  Reversal of cisplatin resistance by the 1,4-benzothiazepine derivative, JTV-519.

Authors:  T Nakamura; F Koizumi; N Kaneko; T Tamura; F Chiwaki; Y Koh; S Akutagawa; N Saijo; K Nishio
Journal:  Jpn J Cancer Res       Date:  2001-06
  2 in total

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