High levels of engraftment with a single infusion of bone marrow cells into normal unprepared mice.

Repetitive infusion of 40 million male murine marrow cells (total 200 million cells) into normal unprepared female BALB/c hosts for 5 consecutive days results in high levels of engraftment at 1-25 months postinfusion, as determined by Southern blot analysis using a Y chromosome-specific probe. We investigated the importance of the schedule of injections in this engraftment model. Surprisingly, a single infusion of 200 x 10(6) male BALB/c bone marrow cells analyzed at 7-14 weeks postinfusion resulted in engraftment levels in individual female mice of over 50% with mean values of 25 +/- 2% for 44 individual transplant points. Engraftment levels in spleen and thymus were 14 +/- 1% and 18 +/- 3%, respectively. Including heparin in the infusion increased engraftment in marrow, spleen, and thymus. Administration of the cells over five or 10 separate infusions, rather than in one injection, did not increase engraftment levels. If the infused bone marrow cells seeded equally between host spleen, thymus, and bone marrow, and if all cells engrafted, the bone marrow engraftment seen here approaches the theoretical maximum. This suggests either a large number of available "niches" or the displacement of host marrow cells by infused marrow. The latter possibility is upheld by cell counts per tibia/femur and total seven-factor HPP-CFC/tibia, which were not increased. These data suggest that a single infusion of marrow homes quantitatively to spleen, thymus, and bone marrow, possibly displacing host cells in the process.